Isolation of a second recombinant human respiratory syncytial virus monoclonal antibody fragment (Fab RSVF2-5) that exhibits therapeutic efficacy in vivo

被引：24

作者：

Crowe, JE

Gilmour, PS

Murphy, BR

Chanock, RM

Duan, LX

Pomerantz, RJ

Pilkington, GR

机构：

[1] NIAID, Infect Dis Lab, NIH, Bethesda, MD 20892 USA

[2] Thomas Jefferson Univ, Jefferson Med Coll, Dept Med,Div Infect Dis, Ctr Human Virol,Dorrance Hamilton Labs, Philadelphia, PA 19107 USA

[3] Intracel Corp, Issaquah, WA USA

来源：

JOURNAL OF INFECTIOUS DISEASES | 1998年 / 177卷 / 04期

关键词：

D O I：

10.1086/517397

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A second human respiratory syncytial virus (RSV)-neutralizing monoclonal antibody was isolated and its binding site was identified. Fab F2-5 is a broadly reactive fusion (F) protein-specific recombinant Fab generated by antigen selection from a random combinatorial library displayed on the surface of filamentous phage. In an in vitro plaque-reduction test, the Fab RSVF2-5 neutralized the infectivity of a variety of field isolates representing viruses of both RSV subgroups A and B. The Fab recognized an antigenic determinant that differed from the only other human anti-F monoclonal antibody (RSV Fab 19) described thus far. A single dose of 4.0 mg of Fab RSVF2-5/kg of body weight administered by inhalation was sufficient to achieve a 2000-fold reduction in pulmonary virus titer in RSV-infected mice. The antigen-binding domain of Fab RSVF2-5 offers promise as part of a prophylactic regimen for RSV infection in humans.

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收藏

页码：1073 / 1076

页数：4

相关论文

共 12 条

[1] CHARACTERIZATION OF 2 ANTIGENIC SITES RECOGNIZED BY NEUTRALIZING MONOCLONAL-ANTIBODIES DIRECTED AGAINST THE FUSION GLYCOPROTEIN OF HUMAN RESPIRATORY SYNCYTIAL VIRUS [J].

ARBIZA, J ;

TAYLOR, G ;

LOPEZ, JA ;

FURZE, J ;

WYLD, S ;

WHYTE, P ;

STOTT, EJ ;

WERTZ, G ;

SULLENDER, W ;

TRUDEL, M ;

MELERO, JA .

JOURNAL OF GENERAL VIROLOGY, 1992, 73 :2225-2234

[2] HUMAN MONOCLONAL FAB FRAGMENTS DERIVED FROM A COMBINATORIAL LIBRARY BIND TO RESPIRATORY SYNCYTIAL VIRUS-F GLYCOPROTEIN AND NEUTRALIZE INFECTIVITY [J].

BARBAS, CF ;

CROWE, JE ;

CABABA, D ;

JONES, TM ;

ZEBEDEE, SL ;

MURPHY, BR ;

CHANOCK, RM ;

BURTON, DR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (21) :10164-10168

[3] NEUTRALIZATION EPITOPES OF THE F-GLYCOPROTEIN OF RESPIRATORY SYNCYTIAL VIRUS - EFFECT OF MUTATION UPON FUSION FUNCTION [J].

BEELER, JA ;

COELINGH, KV .

JOURNAL OF VIROLOGY, 1989, 63 (07) :2941-2950

[4] AN ANTIGENIC ANALYSIS OF RESPIRATORY SYNCYTIAL VIRUS ISOLATES BY A PLAQUE REDUCTION NEUTRALIZATION TEST [J].

COATES, HV ;

ALLING, DW ;

CHANOCK, RM .

AMERICAN JOURNAL OF EPIDEMIOLOGY, 1966, 83 (02) :299-&

[5]

Collins P.L., 1996, FIELDS VIROLOGY, V3, P1313

[6] RECOMBINANT HUMAN RESPIRATORY SYNCYTIAL VIRUS (RSV) MONOCLONAL-ANTIBODY FAB IS EFFECTIVE THERAPEUTICALLY WHEN INTRODUCED DIRECTLY INTO THE LUNGS OF RSV-INFECTED MICE [J].

CROWE, JE ;

MURPHY, BR ;

CHANOCK, RM ;

WILLIAMSON, RA ;

BARBAS, CF ;

BURTON, DR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (04) :1386-1390

[7] A COMPARISON IN CHIMPANZEES OF THE IMMUNOGENICITY AND EFFICACY OF LIVE ATTENUATED RESPIRATORY SYNCYTIAL VIRUS (RSV) TEMPERATURE-SENSITIVE MUTANT VACCINES AND VACCINIA VIRUS RECOMBINANTS THAT EXPRESS THE SURFACE GLYCOPROTEINS OF RSV [J].

CROWE, JE ;

COLLINS, PL ;

LONDON, WT ;

CHANOCK, RM ;

MURPHY, BR .

VACCINE, 1993, 11 (14) :1395-1404

[8] ENHANCED PULMONARY HISTOPATHOLOGY IS OBSERVED IN COTTON RATS IMMUNIZED WITH FORMALIN-INACTIVATED RESPIRATORY SYNCYTIAL VIRUS (RSV) OR PURIFIED F-GLYCOPROTEIN AND CHALLENGED WITH RSV 3-6 MONTHS AFTER IMMUNIZATION [J].

MURPHY, BR ;

SOTNIKOV, AV ;

LAWRENCE, LA ;

BANKS, SM ;

PRINCE, GA .

VACCINE, 1990, 8 (05) :497-502

[9] Recombinant human fab antibody fragments to HIV-1 REV and tat regulatory proteins: Direct selection from a combinatorial phage display library [J].

Pilkington, GR ;

Duan, LX ;

Zhu, MH ;

Keil, W ;

Pomerantz, RJ .

MOLECULAR IMMUNOLOGY, 1996, 33 (4-5) :439-450

[10]

PRINCE GA, 1978, AM J PATHOL, V93, P771