A modular cross-linking approach for exploring protein interactions

被引：144

作者：

Trester-Zedlitz, M

Kamada, K

Burley, SK

Fenyö, D

Chait, BT

Muir, TW

机构：

[1] Rockefeller Univ, Lab Synthet Prot Chem, New York, NY 10021 USA

[2] Rockefeller Univ, Lab Mass Spectrometry & Gaseous Ion Chem, New York, NY 10021 USA

[3] Rockefeller Univ, Lab Mol Biophys, New York, NY 10021 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2003年 / 125卷 / 09期

关键词：

D O I：

10.1021/ja026917a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A method is described for the elucidation of protein-protein interactions using novel cross-linking reagents and mass spectrometry. The method incorporates (1) a modular solid-phase synthetic strategy for generating the cross-linking reagents, (2) enrichment and digestion of cross-linked proteins using microconcentrators, (3) mass spectrometric analysis of cross-linked peptides, and (4) comprehensive computational analysis of the cross-linking data. This integrated approach has been applied to the study of cross-linking between the components of the heterodimeric protein complex negative cofactor 2.

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收藏

页码：2416 / 2425

页数：10

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