Fluorescence polarization assays for high throughput screening of G protein-coupled receptors

被引:35
作者
Banks, P [1 ]
Gosselin, M [1 ]
Prystay, L [1 ]
机构
[1] Adv Bioconcept Co, Montreal, PQ H4P 2R2, Canada
关键词
D O I
10.1177/108705710000500308
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Fluorescence polarization assays in 384-well microtiter plates have been demonstrated, The performance is suitable for high throughput drug screening applications with respect to speed of analysis, displaceable signal, precision, and sensitivity to various reagents. Rank order of potency was maintained relative to [I-125]-ligand filtration assays, and the effects of the highly colored compounds, tartrazine and Chicago Sky Blue, were insignificant on the polarization signal up to a concentration of 1 mu M. These attributes suggest that accurate assessment of drug binding can be obtained.
引用
收藏
页码:159 / 167
页数:9
相关论文
共 6 条
  • [1] Kolb A, 1997, HIGH THROUGHPUT SCRE, P345
  • [2] A time-resolved fluorometric assay for galanin receptors
    Liu, J
    Gallagher, M
    Horlick, RA
    Robbins, AK
    Webb, ML
    [J]. JOURNAL OF BIOMOLECULAR SCREENING, 1998, 3 (03) : 199 - 206
  • [3] A generic particle-based nonradioactive homogeneous multiplex method for high-throughput screening using microvolume fluorimetry
    Martens, C
    Bakker, A
    Rodriguez, A
    Mortensen, RB
    Barrett, RW
    [J]. ANALYTICAL BIOCHEMISTRY, 1999, 273 (01) : 20 - 31
  • [4] Homogeneous cell- and bead-based assays for high throughput screening using fluorometric microvolume assay technology
    Miraglia, S
    Swartzman, EE
    Mellentin-Michelotti, J
    Evangelista, L
    Smith, C
    Gunawan, I
    Lohman, K
    Goldberg, EM
    Manian, B
    Yuan, PM
    [J]. JOURNAL OF BIOMOLECULAR SCREENING, 1999, 4 (04) : 193 - 204
  • [5] A high-throughput STAT binding assay using fluorescence polarization
    Wu, PG
    Brasseur, M
    Schindler, U
    [J]. ANALYTICAL BIOCHEMISTRY, 1997, 249 (01) : 29 - 36
  • [6] A simple statistical parameter for use in evaluation and validation of high throughput screening assays
    Zhang, JH
    Chung, TDY
    Oldenburg, KR
    [J]. JOURNAL OF BIOMOLECULAR SCREENING, 1999, 4 (02) : 67 - 73