Horizontal escape of the novel Tc1-like lepidopteran transposon TCp3.2 into Cydia pomonella granulovirus

被引:60
作者
Jehle, JA
Nickel, A
Vlak, JM
Backhaus, H
机构
[1] Inst Biochem & Pflanzenvirol, Biol Bundesanstalt Land & Forstwirtschaft, D-38104 Braunschweig, Germany
[2] Agr Univ Wageningen, Dept Virol, NL-6700 EM Wageningen, Netherlands
关键词
Cydia pomonella granulovirus; baculovirus; insect transposons; Tc1;
D O I
10.1007/PL00006296
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We characterized an insertion mutant of the baculovirus Cydia pomonella granulovirus (CpGV), which contained a transposable element of 3.2 kb, This transposon, termed TCp3.2, has unusually long inverted terminal repeats (ITRs) of 756 bp and encodes a defective gene for a putative transposase. Amino acid sequence comparison of the defective transposase gene revealed a distant relationship to a putative transposon in Caenorhabditis elegans which also shares some similarity of the ITRs, Maximum parsimony analysis of the predicted amino acid sequences of Tc 1- and mariner-like transposases available from the GenBank data base grouped TCp3.2 within the superfamily of Tc1-like transposons, DNA hybridization indicated that TCp3.2 originated from the genome of Cydia pomonella, which is the natural host of CpGV, and is present in less than 10 copies in the C. pomonella genome. The transposon TCp3.2 most likely was inserted into the viral genome during infection of host larvae. TCp3.2 and the recently characterized Tc1-like transposon TC14.7 (Jehle et al. 1995), which was also found in a CpGV mutant, represent a new family of transposons found in baculovirus genomes, The occasional horizontal escape of different types of host transposons into baculovirus genomes evokes the question about the possible role of baculoviruses as an interspecies vector in the horizontal transmission of insect transposons.
引用
收藏
页码:215 / 224
页数:10
相关论文
共 45 条
[1]   SEQUENCE COMPARISON OF CELLULAR AND VIRAL COPIES OF HOST-CELL DNA INSERTIONS FOUND IN AUTOGRAPHA-CALIFORNICA NUCLEAR POLYHEDROSIS-VIRUS [J].
BEAMES, B ;
SUMMERS, MD .
VIROLOGY, 1990, 174 (02) :354-363
[2]   THE MARINER TRANSPOSABLE ELEMENT IN THE DROSOPHILIDAE FAMILY [J].
BRUNET, F ;
GODIN, F ;
DAVID, JR ;
CAPY, P .
HEREDITY, 1994, 73 :377-385
[3]   EVIDENCE FOR A COMMON EVOLUTIONARY ORIGIN OF INVERTED REPEAT TRANSPOSONS IN DROSOPHILA AND PLANTS - HOBO, ACTIVATOR, AND TAM3 [J].
CALVI, BR ;
HONG, TJ ;
FINDLEY, SD ;
GELBART, WM .
CELL, 1991, 66 (03) :465-471
[4]   BACULOVIRUS-MEDIATED EXPRESSION OF BACTERIAL GENES IN DIPTERAN AND MAMMALIAN-CELLS [J].
CARBONELL, LF ;
KLOWDEN, MJ ;
MILLER, LK .
JOURNAL OF VIROLOGY, 1985, 56 (01) :153-160
[5]   BACULOVIRUS INTERACTION WITH NONTARGET ORGANISMS - A VIRUS-BORNE REPORTER GENE IS NOT EXPRESSED IN 2 MAMMALIAN-CELL LINES [J].
CARBONELL, LF ;
MILLER, LK .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1987, 53 (07) :1412-1417
[7]   TRANSPOSON MUTAGENESIS OF BACULOVIRUSES - ANALYSIS OF TRICHOPLUSIA-NI TRANSPOSON IFP2 INSERTIONS WITHIN THE FP-LOCUS OF NUCLEAR POLYHEDROSIS VIRUSES [J].
CARY, LC ;
GOEBEL, M ;
CORSARO, BG ;
WANG, HG ;
ROSEN, E ;
FRASER, MJ .
VIROLOGY, 1989, 172 (01) :156-169
[8]  
COLLINS J, 1989, GENETICS, V120, P621
[9]   DNA-BINDING ACTIVITIES OF THE CAENORHABDITIS-ELEGANS TC3 TRANSPOSASE [J].
COLLOMS, SD ;
VANLUENEN, HGAM ;
PLASTERK, RHA .
NUCLEIC ACIDS RESEARCH, 1994, 22 (25) :5548-5554
[10]  
DANIELS SB, 1990, GENETICS, V124, P339