Redox buffering of hypochlorous acid by thiocyanate in physiologic fluids

被引：99

作者：

Ashby, MT ^{[1
]}

Carlson, AC ^{[1
]}

Scott, MJ ^{[1
]}

机构：

[1] Univ Oklahoma, Dept Chem & Biochem, Norman, OK 73019 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2004年 / 126卷 / 49期

关键词：

D O I：

10.1021/ja0438361

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The major antimicrobial products of neutrophilic myeloperoxidase (MPO) in physiologic fluids are hypochlorous acid (HOCl) and hypothiocyanite (OSCN-), and the former is generally believed to be the killing agent. However, we have determined that HOCl oxidizes SCN- in a facile nonenzymic reaction. The observed kinetics and computational models substantiate the hypothesis that SCN- serves to moderate the potential autotoxicity of HOCl by restricting its lifetime in physiologic fluids. Furthermore, the oxidizing equivalents of HOCl are preserved in OSCN-, a more discriminate biocide that is not lethal to mammalian cells. Copyright © 2004 American Chemical Society.

引用

页码：15976 / 15977

页数：2

共 17 条

[1]

ALTMAN LC, 1992, J PERIODONTAL RES, V27, P70

[2] Reactive sulfur species: Aqueous chemistry of sulfenyl thiocyanates [J].

Ashby, MT ;

Aneetha, H .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (33) :10216-10217

[3] Formation of trithiocyanate in the oxidation of aqueous thiocyanate [J].

Barnett, JJ ;

Stanbury, DM .

INORGANIC CHEMISTRY, 2002, 41 (02) :164-166

[4]

BJOERCK L, 1980, J DAIRY SCI, V63, P919, DOI 10.3168/jds.S0022-0302(80)83026-8

[5] BACTERICIDAL AND CYTO-TOXIC EFFECTS OF HYPOTHIOCYANITE-HYDROGEN PEROXIDE MIXTURES [J].

CARLSSON, J ;

EDLUND, MB ;

HANSTROM, L .

INFECTION AND IMMUNITY, 1984, 44 (03) :581-586

[6]

CARLSSON J, 1987, J ORAL PATHOL MED, V16, P412

[7]

Conese Massimo, 2003, J Cyst Fibros, V2, P129, DOI 10.1016/S1569-1993(03)00063-8

[8]

DUH SH, 2000, LAB REFERENCE VALUES, P2074

[9] Mechanism of reaction of myeloperoxidase with hydrogen peroxide and chloride ion [J].

Furtmüller, PC ;

Obinger, C ;

Hsuanyu, Y ;

Dunford, HB .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 2000, 267 (19) :5858-5864

[10] Reaction of myeloperoxidase compound I with chloride, bromide, iodide, and thiocyanate [J].

Furtmüller, PG ;

Burner, U ;

Obinger, C .

BIOCHEMISTRY, 1998, 37 (51) :17923-17930

← 1 2 →