Kinetic analysis of calcein and calcein -: Acetoxymethylester efflux mediated by the multidrug resistance protein and P-glycoprotein

被引:172
作者
Essodaïgui, M
Broxterman, HJ
Garnier-Suillerot, A [1 ]
机构
[1] Univ Paris 13, CNRS, URA 2056, Lab Physicochim Biomol & Cellulaire, F-93017 Bobigny, France
[2] Free Univ Amsterdam, Acad Ziekenhuis, Dept Med Oncol, NL-1081 HV Amsterdam, Netherlands
关键词
D O I
10.1021/bi9718043
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Multidrug resistance protein (MRP) and P-glycoprotein (Pgp) are both members of the superfamily of ATP binding cassette plasma membrane drug transport proteins, which may be partly responsible for multidrug resistance of tumor cells. Although MRP has been identified as an organic anion transporter and Pgp as a transporter of certain positively charged compounds, there is considerable overlap in resistance spectrum, suggesting that both proteins transport important anticancer agents such as doxorubicin, etoposide, and vincristine. To obtain more insight in the handling of drugs by both proteins, we performed a derailed kinetic analysis of the efflux of calcein-acetoxymethyl eater (GAL-AM), a common neutral substrate for both proteins and compared it with the kinetics of efflux of calcein (GAL) which is only effluxed by MRP. GAL, the hydrolysis product of the nonfluorescent GAL-AM, is negatively charged and highly fluorescent. For this purpose Pgp+ K562/ADR and MRP+ GLC4/ADR tumor cells were incubated with GAL-AM in ATP-rich or ATP-depleted buffer, and the calcein formation was followed in time by fluorescence development. The intracellular GAL could be distinguished from effluxed (extracellular) CAL by addition to the medium of Co2+, which completely quenched the extracellular CAL fluorescence. The results showed that the V-max,,, for efflux of CAL-AM and GAL by MRP were very similar (1.0-1.2 x 10(5) molecules/cell/s) but that the K-m,for CAL-AM was much lower (0.05 mu M) than for CAL (268 mu M). The latter therefore is much less efficiently transported by MRP than CAL-AM. The K-m for CAL-AM transport by Pgp (0.12 mu M) was similar to that for MRP. Compared to previously published data for anthracyclines, the kinetic data for MRP-mediated CAL-AM pumping are most similar to those for the neutral hydroxydaunorubicin. These data give a quantitative account of transport properties of MRP for two related but differently charged compounds.
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页码:2243 / 2250
页数:8
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