Antigenic characteristics of polypeptides of Coxiella burnetii isolates

被引：18

作者：

To, H

Hotta, A

Zhang, GQ

Nguyen, SV

Ogawa, M

Yamaguchi, T

Fukushi, H

Amano, K

Hirai, K ^{[1
]}

机构：

[1] Gifu Univ, Fac Agr, Dept Vet Microbiol, Yanagido 1-1, Gifu 5011193, Japan

[2] Akita Univ, Cent Res Lab, Akita 0108543, Japan

来源：

MICROBIOLOGY AND IMMUNOLOGY | 1998年 / 42卷 / 02期

关键词：

28-kDa polypeptide; Coxiella burnetii; immunoblotting; immunogenicity;

D O I：

10.1111/j.1348-0421.1998.tb02255.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Eighteen Coxiella burnetii strains from a variety of clinical and geographical sources were screened for antigenic variation of polypeptides by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) coupled with Coomassie brilliant blue (CBB) staining or immunoblotting. These polypeptide profiles showed the greatest variability in the region from 33 to 8.1 kDa, Such differences in the antigenicity of the polypeptides were also recognized by immunoblotting with 15 various mouse anti-C., burnetii antisera, In addition, we detected a polypeptide at about 28 kDa which was immunodominant in strains from human cases of acute Q fever, milk and ticks but not immunogenic in strains from human cases of chronic Q fever, These findings suggest that this polypeptide is a marker to distinguish between acute and chronic strains.

引用

页码：81 / 85

页数：5

共 25 条

[1] THE IMMUNE-RESPONSE TO PHASE-I AND PHASE-II COXIELLA-BURNETII ANTIGENS AS MEASURED BY WESTERN IMMUNOBLOTTING [J].