Characterization of the Aspergillus flavus population within an Illinois maize field

被引:39
作者
Wicklow, DT [1 ]
McAlpin, CE [1 ]
Platis, CE [1 ]
机构
[1] ARS, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA
来源
MYCOLOGICAL RESEARCH | 1998年 / 102卷
关键词
D O I
10.1017/S0953756297004851
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
An evaluation was made of the genotypic diversity (DNA fingerprinting) of 269 A. flavus strains, including subpopulations isolated from grain sampled at harvest (91 unique 'fingerprints' or genotypes/128 strains), field soil (26 genotypes/31 strains), maize insects (49 genotypes/52 strains) and air-spora (56 genotypes/58 strains), from a maize field near Kilbourne, Illinois. Eight A. flavus genotypes were isolated from grain samples harvested in different years (1988-1991). Genotype 36, isolated from three maize samples, matched the DNA fingerprint of a K. E. Papa strain NRRL 19997, isolated from maize grown in Georgia. Ninety-eight percent of the A. flavus genotypes produced sclerotia and 53 % produced aflatoxin. Contrasts of DNA fingerprints revealed two matches involving subpopulations from grain and soil, one match for grain and maize insects, and no matches for grain and air-spora. The high genotypic diversity recorded for each subpopulation, in addition to a limited sample size, precluded any assessment of the relative importance of these subpopulations as sources of A. flavus infective inoculum. Aspergillus parasiticus was routinely isolated from soil samples.
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页码:263 / 268
页数:6
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