The Exo70 Subunit of the Exocyst Is an Effector for Both Cdc42 and Rho3 Function in Polarized Exocytosis

被引:103
作者
Wu, Hao [1 ]
Turner, Courtney [1 ]
Gardner, Jimmy [1 ]
Temple, Brenda [2 ]
Brennwald, Patrick [1 ]
机构
[1] Univ N Carolina, Dept Cell & Dev Biol, Chapel Hill, NC 27599 USA
[2] Univ N Carolina, Dept Biochem & Biophys, Chapel Hill, NC 27599 USA
基金
美国国家卫生研究院;
关键词
SACCHAROMYCES-CEREVISIAE; CRYSTAL-STRUCTURE; PLASMA-MEMBRANE; BUD FORMATION; YEAST; GTPASE; COMPLEX; GROWTH; PHOSPHOLIPIDS; ACTIVATION;
D O I
10.1091/mbc.E09-06-0501
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The Rho3 and Cdc42 members of the Rho GTPase family are important regulators of exocytosis in yeast. However, the precise mechanism by which they regulate this process is controversial. Here, we present evidence that the Exo70 component of the exocyst complex is a direct effector of both Rho3 and Cdc42. We identify gain-of-function mutants in EXO70 that potently suppress mutants in RHO3 and CDC42 defective for exocytic function. We show that Exo70 has the biochemical properties expected of a direct effect or for both Rho3 and Cdc42. Surprisingly, we find that C-terminal prenylation of these GTPases both promotes the interaction and influences the sites of binding within Exo70. Finally, we demonstrate that the phenotypes associated with novel loss-of-function mutants in EXO70, are entirely consistent with Exo70 as an effector for both Rho3 and Cdc42 function in secretion. These data suggest that interaction with the Exo70 component of the exocyst is a key event in spatial regulation of exocytosis by Rho GTPases.
引用
收藏
页码:430 / 442
页数:13
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