Interaction of potassium cyanide with the [Ni-4Fe-5S] active site cluster of CO dehydrogenase from Carboxydothermus hydrogenoformans

The Ni-Fe carbon monoxide ( CO) dehydrogenase II ( CODHIICh) from the anaerobic CO-utilizing hydrogenogenic bacterium Carboxydothermus hydrogenoformans catalyzes the oxidation of CO, presumably at the Ni-(mu S-2)-Fe1 subsite of the [ Ni-4S-5S] cluster in the active site. The CO oxidation mechanism proposed on the basis of several CODHIICh crystal structures involved the apical binding of CO at the nickel ion and the activation of water at the Fe1 ion of the cluster. To understand how CO interacts with the active site, we have studied the reactivity of the cluster with potassium cyanide and analyzed the resulting type of nickel coordination by x-ray absorption spectroscopy. Cyanide acts as a competitive inhibitor of reduced CODHIICh with respect to the substrate CO and is therefore expected to mimic the substrate. It inhibits the enzyme reversibly, forming a nickel cyanide. In this reaction, one of the four square-planar sulfur ligands of nickel is replaced by the carbon atom of cyanide, suggesting removal of the mu S-2 from the Ni-(mu S-2)-Fe1 subsite. Upon reactivation of the inhibited enzyme, cyanide is released, and the square-planar coordination of nickel by 4S ligands is recovered, which includes the reformation of the Ni-(mu S-2)-Fe1 bridge. The results are summarized in a model of the CO oxidation mechanism at the [ Ni-4Fe-5S] active site cluster of CODHIICh from C. hydrogenoformans.