Zinc uptake, oxidative stress and the FNR-like proteins of Lactococcus lactis

被引：28

作者：

Scott, C

Rawsthorne, H

Upadhyay, M

Shearman, CA

Gasson, MJ

Guest, JR

Green, J

机构：

[1] Univ Sheffield, Krebs Inst Biomolec Res, Dept Mol Biol & Biotechnol, Sheffield S10 2TN, S Yorkshire, England

[2] Inst Food Res, Norwich NR4 7UA, Norfolk, England

来源：

FEMS MICROBIOLOGY LETTERS | 2000年 / 192卷 / 01期

关键词：

lactic acid bacterium; zinc; oxidative stress; FNR-like protein; cadmium; thiol protection;

D O I：

10.1111/j.1574-6968.2000.tb09363.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Lactococcus lactis ssp. cremoris MG1363 contains two FNR homologues, FlpA and FlpB, encoded by the distal genes of two paralogous operons (orfX(A/B)-orfY(A/B)-flpA/B). An flpA flpB double mutant strain is hypersensitive to hydrogen peroxide and has a depleted intracellular Zn(II) pool. The phenotypes of the flp mutant strains suggest that FlpA and FlpB control the expression of high and low affinity ATP dependent Zn(II) uptake systems, respectively. Plate tests revealed that expression from a orfX(B)::lac reporter was activated by Cd(II), consistent with other Zn(II)-regulated systems. The link between a failure to acquire Zn(II) and hypersensitivity to oxidative stress suggests that Zn(II) may be required to protect vulnerable protein thiols from oxidation. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

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页码：85 / 89

页数：5

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