Mass spectrometry sampling under ambient conditions with desorption electrospray ionization

被引:2734
作者
Takáts, Z [1 ]
Wiseman, JM [1 ]
Gologan, B [1 ]
Cooks, RG [1 ]
机构
[1] Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA
关键词
D O I
10.1126/science.1104404
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
A new method of desorption ionization is described and applied to the ionization of various compounds, including peptides and proteins present on metal, polymer, and mineral surfaces. Desorption electrospray ionization (DESI) is carried out by directing electrosprayed charged droplets and ions of solvent onto the surface to be analyzed. The impact of the charged particles on the surface produces gaseous ions of material originally present on the surface. The resulting mass spectra are similar to normal ESI mass spectra in that they show mainly singly or multiply charged molecular ions of the analytes. The DESI phenomenon was observed both in the case of conductive and insulator surfaces and for compounds ranging from nonpolar small molecules such as lycopene, the alkaloid coniceine, and small drugs, through polar compounds such as peptides and proteins. Changes in the solution that is sprayed can be used to selectively ionize particular compounds, including those in biological matrices. in vivo analysis is demonstrated.
引用
收藏
页码:471 / 473
页数:3
相关论文
共 23 条
[1]   Impact desolvation of electrosprayed microdroplets - a new ionization method for mass spectrometry of large biomolecules [J].
Aksyonov, SA ;
Williams, P .
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2001, 15 (21) :2001-2006
[2]  
BENNINGHOVEN A, 1987, CHEM ANAL SERIES MON, V86
[3]   CLUSTERS AS PROJECTILES FOR SIMS [J].
BLAIN, MG ;
SCHWEIKERT, EA ;
DASILVEIRA, EF .
JOURNAL DE PHYSIQUE, 1989, 50 (C-2) :85-92
[4]   Profiling and imaging proteins in tissue sections by MS [J].
Chaurand, P ;
Schwartz, SA ;
Caprioli, RM .
ANALYTICAL CHEMISTRY, 2004, 76 (05) :86A-93A
[5]  
CORNETT DS, 1994, RAPID COMMUN MASS SP, V8, P996
[6]   Quantitative determination of noncovalent binding interactions using soft ionization mass spectrometry [J].
Daniel, JM ;
Friess, SD ;
Rajagopalan, S ;
Wendt, S ;
Zenobi, R .
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 2002, 216 (01) :1-27
[7]   MASSIVE CLUSTER-IMPACT IONIZATION ON A 4-SECTOR TANDEM MASS-SPECTROMETER [J].
FABRIS, D ;
WU, Z ;
FENSELAU, CC .
JOURNAL OF MASS SPECTROMETRY, 1995, 30 (01) :140-143
[8]   ELECTROSPRAY IONIZATION-PRINCIPLES AND PRACTICE [J].
FENN, JB ;
MANN, M ;
MENG, CK ;
WONG, SF ;
WHITEHOUSE, CM .
MASS SPECTROMETRY REVIEWS, 1990, 9 (01) :37-70
[9]   ELECTROSPRAY IONIZATION FOR MASS-SPECTROMETRY OF LARGE BIOMOLECULES [J].
FENN, JB ;
MANN, M ;
MENG, CK ;
WONG, SF ;
WHITEHOUSE, CM .
SCIENCE, 1989, 246 (4926) :64-71
[10]   Gas-phase enantioselectivity [J].
Filippi, A ;
Giardini, A ;
Piccirillo, S ;
Speranza, M .
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 2000, 198 (03) :137-163