Type III Interferons Produced by Human Placental Trophoblasts Confer Protection against Zika Virus Infection

被引：427

作者：

Bayer, Avraham ^{[1
,2
]}

Lennemann, Nicholas J. ^{[3
]}

Ouyang, Yingshi ^{[1
,2
]}

Bramley, John C. ^{[3
]}

Morosky, Stefanie ^{[3
]}

De Azeved Marques, Ernesto Torres, Jr. ^{[4
,5
]}

Cherry, Sara ^{[6
]}

Sadovsky, Yoel ^{[1
,2
,3
]}

Coyne, Carolyn B. ^{[1
,2
,3
]}

机构：

[1] Univ Pittsburgh, Magee Womens Res Inst, Pittsburgh, PA 15213 USA

[2] Univ Pittsburgh, Dept Obstet Gynecol & Reprod Sci, Pittsburgh, PA 15213 USA

[3] Univ Pittsburgh, Dept Microbiol & Mol Genet, Pittsburgh, PA 15219 USA

[4] Univ Pittsburgh, Ctr Vaccine Res, Pittsburgh, PA 15261 USA

[5] Fundacao Osvaldo Cruz FIOCRUZ, BR-50670420 Recife, PE, Brazil

[6] Univ Penn, Dept Microbiol, Philadelphia, PA 19104 USA

来源：

CELL HOST & MICROBE | 2016年 / 19卷 / 05期

关键词：

IN-VITRO; LAMBDA; BRAIN; DIFFERENTIATION; BRAZIL; CELLS;

D O I：

10.1016/j.chom.2016.03.008

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

During mammalian pregnancy, the placenta acts as a barrier between the maternal and fetal compartments. The recently observed association between Zika virus (ZIKV) infection during human pregnancy and fetal microcephaly and other anomalies suggests that ZIKV may bypass the placenta to reach the fetus. This led us to investigate ZIKV infection of primary human trophoblasts (PHTs), which are the barrier cells of the placenta. We discovered that PHT cells from full-term placentas are refractory to ZIKV infection. In addition, medium from uninfected PHT cells protects non-placental cells from ZIKV infection. PHT cells constitutively release the type III interferon (IFN) IFN lambda 1, which functions in both a paracrine and autocrine manner to protect trophoblast and non-trophoblast cells from ZIKV infection. Our data suggest that for ZIKV to access the fetal compartment, it must evade restriction by trophoblast- derived IFNl1 and other trophoblast-specific antiviral factors and/or use alternative strategies to cross the placental barrier.

引用

页码：705 / 712

页数：8

共 35 条

[1] Temperature-dependent production of pseudoinfectious dengue reporter virus particles by complementation [J].

Ansarah-Sobrinho, Camilo ;

Nelson, Steevenson ;

Jost, Christiane A. ;

Whitehead, Stephen S. ;

Pierson, Theodore C. .

VIROLOGY, 2008, 381 (01) :67-74

[2]

Bazer FW, 1996, AM J REPROD IMMUNOL, V35, P297

[3] Activation of Type III Interferon Genes by Pathogenic Bacteria in Infected Epithelial Cells and Mouse Placenta [J].

Bierne, Helene ;

Travier, Laetitia ;

Mahlakoiv, Tanel ;

Tailleux, Ludovic ;

Subtil, Agathe ;

Lebreton, Alice ;

Paliwal, Anupam ;

Gicquel, Brigitte ;

Staeheli, Peter ;

Lecuit, Marc ;

Cossart, Pascale .

PLOS ONE, 2012, 7 (06)

[4] Zika Virus Infection in Pregnant Women in Rio de Janeiro [J].

Brasil, P. ;

Pereira, J. P., Jr. ;

Moreira, M. E. ;

Ribeiro Nogueira, R. M. ;

Damasceno, L. ;

Wakimoto, M. ;

Rabello, R. S. ;

Valderramos, S. G. ;

Halai, U. -A. ;

Salles, T. S. ;

Zin, A. A. ;

Horovitz, D. ;

Daltro, P. ;

Boechat, M. ;

Gabaglia, C. Raja ;

Carvalho de Sequeira, P. ;

Pilotto, J. H. ;

Medialdea-Carrera, R. ;

Cotrim da Cunha, D. ;

Abreu de Carvalho, L. M. ;

Pone, M. ;

Machado Siqueira, A. ;

Calvet, G. A. ;

Rodrigues Baiao, A. E. ;

Neves, E. S. ;

Nassar de Carvalho, P. R. ;

Hasue, R. H. ;

Marschik, P. B. ;

Einspieler, C. ;

Janzen, C. ;

Cherry, J. D. ;

Bispo de Filippis, A. M. ;

Nielsen-Saines, K. .

NEW ENGLAND JOURNAL OF MEDICINE, 2016, 375 (24) :2321-2334

[5] Detection and sequencing of Zika virus from amniotic fluid of fetuses with microcephaly in Brazil: a case study [J].

Calvet, Guilherme ;

Aguiar, Renato S. ;

Melo, Adriana S. O. ;

Sampaio, Simone A. ;

de Filippis, Ivano ;

Fabri, Allison ;

Araujo, Eliane S. M. ;

de Sequeira, Patricia C. ;

de Mendonca, Marcos C. L. ;

de Oliveira, Louisi ;

Tschoeke, Diogo A. ;

Schrago, Carlos G. ;

Thompson, Fabiano L. ;

Brasil, Patricia ;

dos Santos, Flavia B. ;

Nogueira, Rita M. R. ;

Tanurit, Amilcar ;

de Filippis, Ana M. B. .

LANCET INFECTIOUS DISEASES, 2016, 16 (06) :653-660

[6] Association between Zika virus and microcephaly in French Polynesia, 2013-15: a retrospective study [J].

Cauchemez, Simon ;

Besnard, Marianne ;

Bompard, Priscillia ;

Dub, Timothee ;

Guillemette-Artur, Prisca ;

Eyrolle-Guignot, Dominique ;

Salje, Henrik ;

Van Kerkhove, Maria D. ;

Abadie, VRonique ;

Garel, Catherine ;

Fontanet, Arnaud ;

Mallet, Henri-Pierre .

LANCET, 2016, 387 (10033) :2125-2132

[7] Human placental trophoblasts confer viral resistance to recipient cells [J].

Delorme-Axford, Elizabeth ;

Donker, Rogier B. ;

Mouillet, Jean-Francois ;

Chu, Tianjiao ;

Bayer, Avraham ;

Ouyang, Yingshi ;

Wang, Tianyi ;

Stolz, Donna B. ;

Sarkar, Saumendra N. ;

Morelli, Adrian E. ;

Sadovsky, Yoel ;

Coyne, Carolyn B. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2013, 110 (29) :12048-12053

[8] Interferon-λ Is Functionally an Interferon but Structurally Related to the Interleukin-10 Family [J].

Gad, Hans Henrik ;

Dellgren, Christoffer ;

Hamming, Ole J. ;

Vends, Susanne ;

Paludan, Soren R. ;

Hartmann, Rune .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2009, 284 (31) :20869-20875

[9] Genetic Characterization of Zika Virus Strains: Geographic Expansion of the Asian Lineage [J].

Haddow, Andrew D. ;

Schuh, Amy J. ;

Yasuda, Chadwick Y. ;

Kasper, Matthew R. ;

Heang, Vireak ;

Huy, Rekol ;

Guzman, Hilda ;

Tesh, Robert B. ;

Weaver, Scott C. .

PLOS NEGLECTED TROPICAL DISEASES, 2012, 6 (02)

[10] PURIFICATION, CHARACTERIZATION, AND INVITRO DIFFERENTIATION OF CYTOTROPHOBLASTS FROM HUMAN TERM PLACENTAE [J].

KLIMAN, HJ ;

NESTLER, JE ;

SERMASI, E ;

SANGER, JM ;

STRAUSS, JF .

ENDOCRINOLOGY, 1986, 118 (04) :1567-1582

← 1 2 3 4 →