Suborganelle Sensing of Mitochondrial cAMP-Dependent Protein Kinase Activity

被引：65

作者：

Agnes, Richard S. ^{[1
]}

Jernigan, Finith ^{[2
,3
]}

Shell, Jennifer R. ^{[2
,3
]}

Sharma, Vyas ^{[2
,3
]}

Lawrence, David S. ^{[2
,3
]}

机构：

[1] Albert Einstein Coll Med, Dept Biochem, Bronx, NY 10461 USA

[2] Univ N Carolina, Dept Chem, Div Med Chem & Nat Prod, Chapel Hill, NC 27599 USA

[3] Univ N Carolina, Dept Pharmacol, Chapel Hill, NC 27599 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2010年 / 132卷 / 17期

关键词：

MAMMALIAN MITOCHONDRIA; REGULATORY SUBUNITS; CATALYTIC SUBUNIT; BINDING; SENSORS; SIGNAL; PHOSPHORYLATION; LOCALIZATION; PROTEOLYSIS; GENOME;

D O I：

10.1021/ja909652q

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A fluorescent sensor of protein kinase activity has been developed and used to characterize the compartmentalized location of cAMP-dependent protein kinase activity in mitochondria. The sensor functions via a phosphorylation-induced release of a quencher from a peptide-based substrate, producing a 150-fold enhancement in fluorescence. The quenching phenomenon transpires via interaction of the quencher with Arg residues positioned on the peptide substrate. Although the cAMP-dependent protein kinase is known to be present in mitochondria, the relative amount of enzyme positioned in the major compartments (outer membrane, intermembrane space, and the matrix) of the organelle is unclear. The fluorescent sensor developed in this study was used to reveal the relative matrix/intermembrane space/outer membrane (85:6:9) distribution of PKA in bovine heart mitochondria.

引用

页码：6075 / 6080

页数：6

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