Use of oxytetracycline and tylosin in intensive calf farming: evaluation of transfer to manure and soil

被引:239
作者
De Liguoro, M [1 ]
Cibin, V
Capolongo, F
Halling-Sorensen, B
Montesissa, C
机构
[1] Univ Padua, Fac Vet Med, Dipartimento Sanita Pubbl Patol Comparata & Igien, I-35020 Legnaro, PD, Italy
[2] Royal Danish Sch Pharm, Dept Analyt & Pharmaceut Chem, DK-2100 Copenhagen, Denmark
关键词
antibiotics; degradation; manure; soil; environmental fate;
D O I
10.1016/S0045-6535(03)00284-4
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Antibiotics may enter soils with manure from treated animals. Because of their biological effects, antibiotics are regarded as potential micropollutants. The levels of oxytetracycline and tylosin over time were followed in faeces, bedding and manure, and then in the soil of a manured field and surrounding drainage courses, after oral treatment of calves. Fifty Simmental calves were treated for 5 days with 60 mg/kg/day of oxytetracycline. After 15 days the animals were treated for 5 days with 20 mg/kg/day of tylosin. Tylosin degraded rapidly, and was no longer detected in manure 45 days after cessation of treatment and no trace of the compound was detected in soil or surrounding water (detection limits 10 mug/l). The half-life of oxytetracycline in manure was 30 days and the compound was still detectable in this matrix (820 mug/kg) after 5 months maturation. In the manured soil oxytetracycline was detected at concentrations at least 10 times lower than the European Agency for the Evaluation of Medicinal Products threshold (100 mug/kg) requiring phase II environmental risk assessment. Oxytetracycline was not detected in the water courses (detection limit 1 mug/l). These results demonstrate that the processes occurring between faeces production and application of manure to the soil are very effective in reducing the load of TYL and OTC in the environment. For both drugs a toxicity test was performed using the alga Selenastrum capricornutum. The EC50 was 4.18 mg/l for oxytetracycline and 0.95 mg/l for tylosin. A worst-case hazard assessment for the aquatic environment was performed comparing the ratio between the measured concentrations (LOD) and effect data from previous work (OTC) or from this work (TYL). This showed ratio between toxicity levels (bacteria) (EC50 = 0.14 mg/l) and measured concentrations (LOD = 1 mug/l) for OTC to be 140. The corresponding value for TYL (LOD = 10 mug/l) was 95. (C) 2003 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:203 / 212
页数:10
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