On-chip contactless four-electrode conductivity detection for capillary electrophoresis devices

被引:88
作者
Laugere, F [1 ]
Guijt, RM
Bastemeijer, J
van der Steen, G
Berthold, A
Baltussen, E
Sarro, P
van Dedem, GWK
Vellekoop, M
Bossche, A
机构
[1] Delft Univ Technol, Dept Microelect, Elect Instrumentat Lab DIMES, Delft, Netherlands
[2] Delft Univ Technol, Fac Sci Appl, Kluyver Lab Biotechnol, Sect Analyt Biotechnol, Delft, Netherlands
[3] Delft Univ Technol, Fac Elect Engn, DIMES, ECTM, Delft, Netherlands
[4] Vienna Univ Technol, Inst Ind Elect & Mat Sci, Vienna, Austria
关键词
D O I
10.1021/ac0157371
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
In this contribution, a capillary electrophoresis microdevice with an integrated on-chip contactless four-electrode conductivity detector is presented. A 6-cm-long, 70-mum-wide, and 20-mum-deep channel was etched in a glass substrate that was bonded to a second glass substrate in order to form a sealed channel. Four contactless electrodes (metal electrodes covered by 30-nm silicon carbide) were deposited and patterned on the second glass substrate for on-chip conductivity detection. Contactless conductivity detection was performed in either a two- or a four-electrode configuration. Experimental results confirmed the improved characteristics of the four-electrode configuration over the classical two-electrode detection setup. The four-electrode configuration allows for sensitive detection for varying carrier-electrolyte background conductivity without the need for adjustment of the measurement frequency. Reproducible electrophoretic separations of three in organic cations (K+, Na+, Li+) and six organic acids are presented. Detection as low as 5 muM for potassium was demonstrated.
引用
收藏
页码:306 / 312
页数:7
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