Bisaryl Hydrazones as Exchangeable Biocompatible Linkers

被引：89

作者：

Dirksen, Anouk ^{[1
,2
]}

Yegneswaran, Subramanian ^{[3
]}

Dawson, Philip E. ^{[1
,2
]}

机构：

[1] Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA

[2] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA

[3] Scripps Res Inst, Dept Mol & Expt Med, La Jolla, CA 92037 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2010年 / 49卷 / 11期

关键词：

affinity purification; bioorganic chemistry; cleavable linkers; homogeneous catalysis; hydrazones; TISSUE FACTOR PATHWAY; BIOTIN-BINDING; NUCLEOPHILIC CATALYSIS; PROTEIN; STREPTAVIDIN; AVIDIN; COMPLEXES; CHEMISTRY; RECOVERY; PROBE;

D O I：

10.1002/anie.200906756

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Catch and release: Stable bisaryl hydrazones undergo rapid exchange under mild conditions in the presence of a catalyst (see schematic illustration; b=biotin). This highly efficient hydrazone exchange is useful for the affinity purification of biotinylated proteins on (strept)avidin beads. The method enables the mild elution of label-exchanged or chemically functionalized proteins. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：2023 / 2027

页数：5

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