Engineering synthetic signaling proteins with ultrasensitive input/output control

被引：108

作者：

Dueber, John E.

Mirsky, Ethan A.

Lim, Wendell A.

机构：

[1] Univ Calif San Francisco, Dept Mol & Cellular Pharmacol, San Francisco, CA 94158 USA

[2] Univ Calif San Francisco, Program Biol Sci, San Francisco, CA 94158 USA

[3] Univ Calif San Francisco, Grad Grp Biophys, San Francisco, CA 94158 USA

[4] Univ Calif San Francisco, UC Berkeley, Nanomed Dev Ctr, San Francisco, CA 94158 USA

[5] NSF Synth Biol Engn Res Ctr, Berkeley, CA 94720 USA

来源：

NATURE BIOTECHNOLOGY | 2007年 / 25卷 / 06期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

D O I：

10.1038/nbt1308

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Many signaling proteins are built from simple, modular components, yet display highly complex signal-processing behavior. Here we explore how modular domains can be used to build an ultrasensitive switch-a nonlinear input/output function that is central to many complex biological behaviors. By systematically altering the number and affinity of modular autoinhibitory interactions, we show that we can predictably convert a simple linear signaling protein into an ultrasensitive switch.

引用

页码：660 / 662

页数：3

共 12 条

[1] The JNK cascade as a biochemical switch in mammalian cells: Ultrasensitive and all-or-none responses [J].