Latest developments in sample treatment for 18O-isotopic labeling for proteomics mass spectrometry-based approaches: A critical review

被引：40

作者：

Capelo, J. L. ^{[1
,2
]}

Carreira, R. J. ^{[2
]}

Fernandes, L. ^{[2
]}

Lodeiro, C. ^{[2
,3
]}

Santos, H. M. ^{[2
]}

Simal-Gandara, J. ^{[1
]}

机构：

[1] Univ Vigo, Fac Food Sci & Technol, Analyt & Food Chem Dept, Nutr & Bromatol Grp, E-32004 Orense, Spain

[2] Univ Nova Lisboa, Dept Quim, REQUIMTE, Fac Ciencias & Tecnol, P-2829516 Monte De Caparica, Portugal

[3] Univ Vigo, Fac Sci, Dept Phys Chem, E-32004 Orense, Spain

来源：

TALANTA | 2010年 / 80卷 / 04期

关键词：

Proteomics; Review; O-18; Isotopic labeling; Protein quantification; PROTEIN IDENTIFICATION; O-18; QUANTIFICATION; DIGESTION; TOOL; CHROMATOGRAPHY; PROTEOLYSIS; PEPTIDES; EXCHANGE; TRYPSIN;

D O I：

10.1016/j.talanta.2009.04.053

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Nowadays isotopic O-18-labeling of peptides has recalled the attention of researchers due to its simplicity of application and high versatility for proteomics studies. Protein quantification, differential peptide mass mapping, studies regarding proteins overexpressed or underexpressed, or the searching of biomarkers can be accomplished by using O-18-labeling. In this critical review we comment on the different ways in which O-18-labeling can be done, highlighting the key parameters of the different sample treatments to obtain a reliable and reproducible labeling. In addition we describe and compare the latest improvement in terms of sample treatment that allows to reduce the handling and to increase the throughput for this sample treatment. Finally, we hypothesize on the future trends of these methods under the light of the new technological advances to speed protein cleavage. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：1476 / 1486

页数：11

共 52 条

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