Induction of immunological memory in UK infants by a meningococcal A/C conjugate vaccine

The induction of immunological memory to serogroup A and C polysaccharides in UK infants immunized with three doses of a meningococcal A/C oligosaccharide CRM197 conjugate vaccine was investigated. Forty UK infants vaccinated previously with three doses of a meningococcal A/C oligosaccharide-CRM197 conjugate vaccine at 2, 3 and 4 months of age, were revaccinated at a mean age of 145.6 weeks with either a 10 or 50 mu g dose of licensed meningococcal A/C polysaccharide vaccine. Serogroup-specific antibody and serum bactericidal antibody (SBA) responses were measured by enzyme-linked immunosorbent assay and serum bactericidal assays, respectively. Following challenge, anti-serogroup A and C polysaccharide antibody levels rose from pro-booster geometric mean concentrations (GMC) of 3.1 and 2.1 mu g/ml respectively to 19.6 and 21.0 mu g/ml 1 month post-booster. Serum bactericidal antibody geometric mean titres (GMTs) for serogroups A and C increased 156- and 113-fold from 2.1 and 7.1 pro-booster respectively to 327.4 and 800.7 post-booster. A serogroup A control group of 45 children received a 10 mu g dose of licensed meningococcal A/C polysaccharide vaccine (with no prior history of serogroup A vaccination) had serogroup A SEA GMTs of 2.3 prevaccination rising to 8 post-vaccination with corresponding GMCs of 0.8 and 10.8 mu g/ml. These rises in SEA following serogroup A/C conjugate vaccination are indicative of immunological priming.