Uncoupling of Expression of an Intronic MicroRNA and Its Myosin Host Gene by Exon Skipping

被引：125

作者：

Bell, Matthew L. ^{[1
]}

Buvoli, Massimo ^{[1
]}

Leinwand, Leslie A. ^{[1
]}

机构：

[1] Univ Colorado, Dept Mol Cellular & Dev Biol, Boulder, CO 80309 USA

来源：

MOLECULAR AND CELLULAR BIOLOGY | 2010年 / 30卷 / 08期

关键词：

SKELETAL-MUSCLE; FATE DETERMINATION; FEEDBACK LOOP; IN-VITRO; SOX6; DIFFERENTIATION; PROTEINS; CELLS; HYPERTROPHY; HEART;

D O I：

10.1128/MCB.01370-09

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

The ancient MYH7b gene, expressed in striated muscle and brain, encodes a sarcomeric myosin and the intronic microRNA miR-499. We find that skipping of an exon introduces a premature termination codon in the transcript that downregulates MYH7b protein production without affecting microRNA expression. Among other genes, endogenous miR-499 targets the 3' untranslated region of the transcription factor Sox6, which in turn acts as a repressor of MYH7b transcriptional activity. Thus, concerted transcription and alternative splicing uncouple the level of expression of MYH7b and miR-499 when their coexpression is not required.

引用

页码：1937 / 1945

页数：9

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