Structural analysis of the lipopolysaccharide from Vibrio cholerae serotype O22

被引：19

作者：

Cox, AD ^{[1
]}

Brisson, JR ^{[1
]}

Thibault, P ^{[1
]}

Perry, MB ^{[1
]}

机构：

[1] Natl Res Council Canada, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada

来源：

CARBOHYDRATE RESEARCH | 1997年 / 304卷 / 3-4期

关键词：

Vibrio cholerae O22; LPS; NMR; mass spectrometry;

D O I：

10.1016/S0008-6215(97)00207-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The structure of the lipopolysaccharide (LPS) from Vibrio cholerae serogroup 022 was elucidated. The LPS was subjected to a variety of degradative procedures, and the structures of the purified products were established by monosaccharide and methylation analyses, nuclear magnetic resonance (NMR) spectroscopy, and mass spectrometry. The following structure for the complete LPS molecule was determined on the basis of the combined data from these experiments. [GRAPHICS] The elucidation of this structure provided a chemical basis for the serological cross-reactions observed between this strain and V. cholerae serogroup 0139. (C) 1997 Elsevier Science Ltd. All rights reserved.

引用

页码：191 / 208

页数：18

共 41 条

[1]

ALBERT MJ, 1993, LANCET, V341, P704

[2] PREDICTION OF ANTI AND GAUCHE VICINAL PROTON-PROTON COUPLING-CONSTANTS IN CARBOHYDRATES - A SIMPLE ADDITIVITY RULE FOR PYRANOSE RINGS [J].

ALTONA, C ;

HAASNOOT, CAG .

ORGANIC MAGNETIC RESONANCE, 1980, 13 (06) :417-429

[3]

Bateman KP, 1997, RAPID COMMUN MASS SP, V11, P307

[4] GENESIS OF THE NOVEL EPIDEMIC VIBRIO-CHOLERAE-0139 STRAIN - EVIDENCE FOR HORIZONTAL TRANSFER OF GENES INVOLVED IN POLYSACCHARIDE SYNTHESIS [J].

BIK, EM ;

BUNSCHOTEN, AE ;

GOUW, RD ;

MOOI, FR .

EMBO JOURNAL, 1995, 14 (02) :209-216

[5] Genetic organization and functional analysis of the otn DNA essential for cell-wall polysaccharide synthesis in Vibrio cholerae O139 [J].

Bik, EM ;

Bunschoten, AE ;

Willems, RJL ;

Chang, ACY ;

Mooi, FR .

MOLECULAR MICROBIOLOGY, 1996, 20 (04) :799-811

[6] C-13 NUCLEAR MAGNETIC-RESONANCE SPECTROSCOPY OF MONOSACCHARIDES [J].

BOCK, K ;

PEDERSEN, C .

ADVANCES IN CARBOHYDRATE CHEMISTRY AND BIOCHEMISTRY, 1983, 41 :27-66

[7] C-13 NUCLEAR MAGNETIC-RESONANCE DATA FOR OLIGOSACCHARIDES [J].

BOCK, K ;

PEDERSEN, C ;

PEDERSEN, H .

ADVANCES IN CARBOHYDRATE CHEMISTRY AND BIOCHEMISTRY, 1984, 42 :193-225

[8] SYNTHESIS AND ACIDIC DEGRADATION OF 3-DEOXY-KETOALDONIC ACIDS [J].

CHARON, D ;

SZABO, L .

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1, 1973, (11) :1175-1179

[9] Cloning and sequence of a region encoding a surface polysaccharide of Vibrio cholerae O139 and characterization of the insertion site in the chromosome of Vibrio cholerae O1 [J].

Comstock, LE ;

Johnson, JA ;

Michalski, JM ;

Morris, JG ;

Kaper, JB .

MOLECULAR MICROBIOLOGY, 1996, 19 (04) :815-826

[10] Structural analysis of the O-antigen-core region of the lipopolysaccharide from Vibrio cholerae O139 [J].

Cox, AD ;

Perry, MB .

CARBOHYDRATE RESEARCH, 1996, 290 (01) :59-65

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