Quantitative cerebral blood flow with Optical Coherence Tomography

被引:188
作者
Srinivasan, Vivek J. [1 ]
Sakadzic, Sava [1 ]
Gorczynska, Iwona [2 ,3 ]
Ruvinskaya, Svetlana [1 ]
Wu, Weicheng [1 ]
Fujimoto, James G. [2 ,3 ]
Boas, David A. [1 ]
机构
[1] Harvard Univ, Massachusetts Gen Hosp, Photon Migrat Imaging Lab,Sch Med, MGH MIT HMS Athinoula A Martinos Ctr Biomed Imagi, Charlestown, MA 02129 USA
[2] MIT, Dept Elect Engn & Comp Sci, Cambridge, MA 02139 USA
[3] MIT, Elect Res Lab, Cambridge, MA 02139 USA
来源
OPTICS EXPRESS | 2010年 / 18卷 / 03期
基金
美国国家卫生研究院;
关键词
IN-VIVO; DOPPLER TOMOGRAPHY; MICRO-ANGIOGRAPHY; SCATTERING MEDIA; RAT NEOCORTEX; PERFUSION; VELOCITY; SPECKLE; HEMODYNAMICS; ARTIFACTS;
D O I
10.1364/OE.18.002477
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
Absolute measurements of cerebral blood flow (CBF) are an important endpoint in studies of cerebral pathophysiology. Currently no accepted method exists for in vivo longitudinal monitoring of CBF with high resolution in rats and mice. Using three-dimensional Doppler Optical Coherence Tomography and cranial window preparations, we present methods and algorithms for regional CBF measurements in the rat cortex. Towards this end, we develop and validate a quantitative statistical model to describe the effect of static tissue on velocity sensitivity. This model is used to design scanning protocols and algorithms for sensitive 3D flow measurements and angiography of the cortex. We also introduce a method of absolute flow calculation that does not require explicit knowledge of vessel angles. We show that OCT estimates of absolute CBF values in rats agree with prior measures by autoradiography, suggesting that Doppler OCT can perform absolute flow measurements in animal models. (C) 2010 Optical Society of America
引用
收藏
页码:2477 / 2494
页数:18
相关论文
共 42 条
[1]   MODEL FOR LASER DOPPLER MEASUREMENTS OF BLOOD-FLOW IN TISSUE [J].
BONNER, R ;
NOSSAL, R .
APPLIED OPTICS, 1981, 20 (12) :2097-2107
[2]   Measuring cerebral blood flow using magnetic resonance imaging techniques [J].
Calamante, F ;
Thomas, DL ;
Pell, GS ;
Wiersma, J ;
Turner, R .
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM, 1999, 19 (07) :701-735
[3]   Optical Doppler tomographic imaging of fluid flow velocity in highly scattering media [J].
Chen, ZP ;
Milner, TE ;
Dave, D ;
Nelson, JS .
OPTICS LETTERS, 1997, 22 (01) :64-66
[4]   Spectral-domain phase microscopy [J].
Choma, MA ;
Ellerbee, AK ;
Yang, CH ;
Creazzo, TL ;
Izatt, JA .
OPTICS LETTERS, 2005, 30 (10) :1162-1164
[5]   Dynamic imaging of cerebral blood flow using laser speckle [J].
Dunn, AK ;
Bolay, T ;
Moskowitz, MA ;
Boas, DA .
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM, 2001, 21 (03) :195-201
[6]   FLOW VISUALIZATION BY MEANS OF SINGLE-EXPOSURE SPECKLE PHOTOGRAPHY [J].
FERCHER, AF ;
BRIERS, JD .
OPTICS COMMUNICATIONS, 1981, 37 (05) :326-330
[7]   Neurovascular coupling in the normal brain and in hypertension, stroke, and Alzheimer disease [J].
Girouard, H ;
Iadecola, C .
JOURNAL OF APPLIED PHYSIOLOGY, 2006, 100 (01) :328-335
[8]   DYNAMIC PENUMBRA DEMONSTRATED BY SEQUENTIAL MULTITRACER PET AFTER MIDDLE CEREBRAL-ARTERY OCCLUSION IN CATS [J].
HEISS, WD ;
GRAF, R ;
WIENHARD, K ;
LOTTGEN, J ;
SAITO, R ;
FUJITA, T ;
ROSNER, G ;
WAGNER, R .
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM, 1994, 14 (06) :892-902
[9]   OPTICAL COHERENCE MICROSCOPY IN SCATTERING MEDIA [J].
IZATT, JA ;
HEE, MR ;
OWEN, GM ;
SWANSON, EA ;
FUJIMOTO, JG .
OPTICS LETTERS, 1994, 19 (08) :590-592
[10]   In vivo bidirectional color Doppler flow imaging of picoliter blood volumes using optical coherence tomograghy [J].
Izatt, JA ;
Kulkami, MD ;
Yazdanfar, S ;
Barton, JK ;
Welch, AJ .
OPTICS LETTERS, 1997, 22 (18) :1439-1441