Genetic analysis of regions involved in replication and cadmium resistance of the plasmid pND302 from Lactococcus lactis

被引：42

作者：

Liu, CQ ^{[1
]}

Khunajakr, N ^{[1
]}

Chia, LG ^{[1
]}

Deng, YM ^{[1
]}

Charoenchai, P ^{[1
]}

Dunn, NW ^{[1
]}

机构：

[1] Univ New S Wales, Dept Biotechnol, Cooperat Res Ctr Food Ind Innovat, Sydney, NSW 2052, Australia

来源：

PLASMID | 1997年 / 38卷 / 02期

关键词：

D O I：

10.1006/plas.1997.1301

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The 8.8-kb Lactococcus lactis plasmid pND302 encodes resistance to cadmium (Cd-R). Regions of pND302 involved in replication and Cd-R were subcloned and sequenced The replication region is localized on a 1.5-kb region and consists of an open reading frame (repB) preceded by a noncoding AT-rich sequence (on) which is highly homologous to lactococcal theta-type replicons. The Cd-R determinant is localized on a 2.9-kb region and encodes putative proteins similar to the Cd2+-specific P-type efflux ATPase (CadA) and the transcriptional regulatory repressor (CadC) identified in Staphylococcus aureus, Bacillus firmus, and Listeria monocytogenes. Similar Cd-R determinants were also detected by PCR in other Cd-R plasmids isolated from different L. lactis strains. (C) 1997 Academic Press.

引用

页码：79 / 90

页数：12

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