Expression of an antisense 1-aminocyclopropane-1-carboxylate oxidase gene stimulates shoot regeneration in Cucumis melo

被引：16

作者：

Amor, MB ^{[1
]}

Guis, M ^{[1
]}

Latché, A ^{[1
]}

Bouzayen, M ^{[1
]}

Pech, JC ^{[1
]}

Roustan, JP ^{[1
]}

机构：

[1] Ecole natl Super Agron Toulouse, INRA, UA, F-31076 Toulouse, France

来源：

PLANT CELL REPORTS | 1998年 / 17卷 / 6-7期

关键词：

Cucumis melo; 1-aminocyclopropane-1-carboxylate oxidase; ethylene; antisense RNA; transgenic plant;

D O I：

10.1007/s002990050447

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The role of ethylene in shoot regeneration was investigated using transgenic Cucumis melo plants expressing an antisense 1-aminocyclopropane-1-carboxylate (ACC) oxidase gene. ACC oxidase catalyses the last step of ethylene biosynthesis. Leaf and cotyledon explants from the transgenic plants exhibited low ACC oxidase activity and ethylene production, whereas the regeneration capacity of the tissues was greatly enhanced (3.5- and 2.8-fold, respectively) compared to untransformed control tissues. Addition of ethylene released by 50 or 100 mu M 2-chloroethylphosphonic acid dramatically reduced the shoot regeneration rate of the transgenic tissues. The results clearly demonstrate that ethylene plays an important role in C. melo morphogenesis in vitro.

引用

页码：586 / 589

页数：4

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