Experimental acute pancreatitis results in increased blood-brain barrier permeability in the rat:: a potential role for tumor necrosis factor and interleukin 6

被引:65
作者
Farkas, G
Márton, J
Nagy, Z
Mándi, Y
Takács, T
Deli, MA
Abrahám, CS
机构
[1] Albert Szent Gyorgyi Med Univ, Dept Surg, Sch Med, H-6701 Szeged, Hungary
[2] Albert Szent Gyorgyi Med Univ, Dept Microbiol, Sch Med, H-6701 Szeged, Hungary
[3] Albert Szent Gyorgyi Med Univ, Dept Med, Sch Med, H-6701 Szeged, Hungary
[4] Biol Res Ctr, Inst Biophys, Mol Neurobiol Lab, H-6701 Szeged, Hungary
关键词
acute pancreatitis; blood-brain barrier; brain edema; encephalopathy; interleukin; 6; tumor necrosis factor;
D O I
10.1016/S0304-3940(98)00060-3
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Pancreatic encephalopathy is a severe complication of acute pancreatitis. Proinflammatory cytokines may play a role in the development of multi-organ failure during pancreatitis. In the present study, we measured the changes in the blood-brain barrier (BBB) permeability concomitantly with the determination of serum tumor necrosis factor (TNF) and interleukin-6 (IL-6) levels in rats before, as well as 6, 24 and 48 h after the beginning of intraductal taurocholic acid-induced acute pancreatitis. Cytokine concentrations were measured in bioassays with specific cell lines (WEHI-164 for TNF and B-9 for IL-6), while the BBB permeability was determined for a small (sodium fluorescein, molecular weight (MW) 376 Da), and a large (Evans' blue-albumin, MW 67 000 Da) tracer by spectrophotometry in the parietal cortex, hippocampus, striatum, cerebellum and medulla of rats. The serum TNF level was significantly (P < 0.05) increased 6 and 24 h after the induction of pancreatitis, while the IL-6 level increased after 24 and 48 h. A significant (P < 0.05) increase in BBB permeability for both tracers developed at 6 and 24 h in different brain regions of animals with acute pancreatitis. We conclude that cytokines, such as TNF and IL-6, may contribute to the vasogenic brain edema formation during acute pancreatitis. (C) 1998 Elsevier Science Ireland Ltd.
引用
收藏
页码:147 / 150
页数:4
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