Immune responses of swine following DNA immunization with plasmids encoding porcine reproductive and respiratory syndrome virus ORFs 5 and 7, and porcine IL-2 and IFNγ

被引：43

作者：

Xue, QN ^{[1
]}

Zhao, YG ^{[1
]}

Zhou, YJ ^{[1
]}

Qiu, HJ ^{[1
]}

Wang, YF ^{[1
]}

Wu, DL ^{[1
]}

Tian, ZJ ^{[1
]}

Tong, GZ ^{[1
]}

机构：

[1] CAAS, Harbin Vet Res Inst, Natl Key Lab Vet Biotechnol, Harbin 150001, Peoples R China

来源：

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY | 2004年 / 102卷 / 03期

基金：

中国国家自然科学基金;

关键词：

porcine reproductive and respiratory syndrome virus (PRRSV); DNA vaccine; porcine IL-2; porcine interferon;

D O I：

10.1016/j.vetimm.2004.09.021

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

In the present study, five eukaryotic double-gene expression plasmids containing porcine reproductive and respiratory syndrome virus (PRRSV) ORF5 and ORF7 genes combined with cDNAs encoding porcine IFNgamma and IL-2 were constructed for evaluation as PRRSV vaccine candidates. After immunization and viral challenge, two of three pigs immunized with pIRESorf5/ IFNgamma, one of three pigs immunized with pIRESorf5/IL-2 and one of three pigs immunized with pIRESorf7IL-2 were protected from lung lesions that were present in other vaccinated and control animals. Virus replication was reduced but not completely prevented in organs of the DNA-vaccinated animals as compared to controls. Therefore, the porcine cytokines IFNgamma and IL-2, delivered in combination with ORF5 or ORF7, may improve the immune efficacy of DNA vaccines against PRRSV. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：291 / 298

页数：8

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