Accurate height and volume measurements on soft samples with the atomic force microscope

被引：83

作者：

Jiao, Y

Schäffer, TE

机构：

[1] Univ Munster, Ctr Nanotechnol, D-48149 Munster, Germany

[2] Univ Munster, Inst Phys, D-48149 Munster, Germany

[3] Max Planck Inst Biophys Chem, Dept Biol Mol, D-37077 Gottingen, Germany

来源：

LANGMUIR | 2004年 / 20卷 / 23期

关键词：

D O I：

10.1021/la048650u

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The suitability of three common atomic force microscope (AFM) imaging modes for quantitative height and volume measurements on soft samples was investigated. The height and volume of rehydrated human metaphase chromosomes in liquid were measured using the contact mode, the tapping mode, and the force mapping mode. In both the contact and tapping modes, the measured height and volume strongly depended on the imaging setpoint that sets the imaging force. Measurement deviations up to 50% were observed. The force mapping mode, on the other hand, yielded reproducible height and volume measurements independent of the imaging force. It is therefore suggested that the force mapping mode should be used whenever the height or volume of soft samples need to be accurately determined.

引用

页码：10038 / 10045

页数：8

共 60 条

[1] Cantilever dynamics in quasinoncontact force microscopy: Spectroscopic aspects [J].