Mufti-grams scale purification of xanthanolides from Xanthium macrocarpum -: Centrifugal partition chromatography versus silica gel chromatography

被引：33

作者：

Pinel, Benoit

Audo, Gregoire

Mallet, Sabine

Lavault, Marie

De La Poype, Francois

Seraphin, Denis

Richomme, Pascal

机构：

[1] UFR Sci Pharmaceut & Ingn Sante, Lab SONAS EA 921, F-49100 Angers, France

[2] Kromaton SARL, F-49000 Angers, France

来源：

JOURNAL OF CHROMATOGRAPHY A | 2007年 / 1151卷 / 1-2期

关键词：

centrifugal partition chromatography; xanthanolide; sesquiterpene lactone; Xanthium macrocarpum;

D O I：

10.1016/j.chroma.2007.02.115

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Plants of the Asteraceae family are known as a source of sesquiterpene lactones with interesting biological activities. The purification of several xanthanolides (xanthathin, 4-epi-xanthanol and 4-epi-isoxanthanol) was realized in one step, directly from the crude chloroformic extract of the leaves of X. macrocarpum by silica gel chromatography and for the first time by liquid/liquid chromatography (counter-current chromatography/centrifugal partition chromatography), using a FCPC 5L (Fast centrifugal partition chromatograph, Kromaton Technologies, Angers, France). Recovery, purity of xanthathin and solvent consumption were improved with the liquid/liquid chromatography compared to solid/liquid chromatography. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：14 / 19

页数：6

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