Six years of in situ CO2 enrichment evoke changes in soil structure and soil biota of nutrient-poor grassland

Nutrient-poor grassland on a silty clay loam overlying calcareous debris was exposed to elevated CO(2) for six growing seasons. The CO(2) exchange and productivity were persistently increased throughout the experiment, suggesting increases in soil C inputs. At the same time, elevated CO(2) lead to increased soil moisture due to reduced evapotransporation. Measurements related to soil microflora did not indicate increased soil C fluxes under elevated CO(2). Microbial biomass, soil basal respiration, and the metabolic quotient for CO(2) (qCO(2)) were not altered significantly. PLFA analysis indicated no significant shift in the ratio of fungi to bacteria. 0.5 m KCl extractable organic C and N, indicators of changed DOC and DON concentrations, also remained unaltered. Microbial grazer populations (protozoa, bacterivorous and fungivorous nematodes, acari and collembola) and root feeding nematodes were not affected by elevated CO(2). However, total nematode numbers averaged slightly lower under elevated CO(2) (-16%, ns) and nematode mass was significantly reduced (-43%, P = 0.06). This reduction reflected a reduction in large-diameter nematodes classified as omnivorous and predacious. Elevated CO(2) resulted in a shift towards smaller aggregate sizes at both micro- and macro-aggregate scales; this was caused by higher soil moisture under elevated CO(2). Reduced aggregate sizes result in reduced pore neck diameters. Locomotion of large-diameter nematodes depends on the presence of large enough pores; the reduction in aggregate sizes under elevated CO(2) may therefore account for the decrease in large nematodes. These animals are relatively high up the soil food web; this decline could therefore trigger top-down effects on the soil food web. The CO(2) enrichment also affected the nitrogen cycle. The N stocks in living plants and surface litter increased at elevated CO(2), but N in soil organic matter and microbes remained unaltered. Nitrogen mineralization increased markedly, but microbial N did not differ between CO(2) treatments, indicating that net N immobilization rates were unaltered. In summary, this study did not provide evidence that soils and soil microbial communities are affected by increased soil C inputs under elevated CO(2). On the contrary, available data ((13)C tracer data, minirhizotron observations, root ingrowth cores) suggests that soil C inputs did not increase substantially. However, we provide first evidence that elevated CO(2) can reduce soil aggregation at the scale from mum to mm scale, and that this can affect soil microfaunal populations.