ANG II controls Na+-K+(NH+4)-2Cl- cotransport via 20-HETE and PKC in medullary thick ascending limb

被引:66
作者
Amlal, H
LeGoff, C
Vernimmen, C
Soleimani, M
Paillard, M
Bichara, M
机构
[1] Univ Paris 06, Ctr Rech Biomed Cordeliers, INSERM, U356, F-75270 Paris 06, France
[2] Univ Cincinnati, Sch Med, Dept Internal Med & Mol Genet, Cincinnati, OH 45267 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY | 1998年 / 274卷 / 04期
关键词
intracellular pH; intracellular calcium; cytochrome P-450-dependent monooxygenase; phospholipase C; diacylglycerol lipase; protein kinase C; angiotensin II receptors; DUP-753; PD-123319;
D O I
10.1152/ajpcell.1998.274.4.C1047
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Cell pH was monitored in medullary thick ascending limbs to determine effects of ANG II on Na+-K+(NH4+)-2Cl(-) cotransport. ANG II at 10(-16) to 10(-12) M inhibited 30-50% (P < 0.005), but higher ANG II concentrations were stimulatory compared with the 10(-12) M ANG II level cotransport activity; eventually, 10(-6) M ANG II stimulated 34% cotransport activity (P < 0.003). Inhibition by 10(-12) M ANG II was abolished by phospholipase C (PLC), diacylglycerol lipase, or cytochrome P-450-dependent monooxygenase blockade; 10(-12) M ANG II had no effect additive to inhibition by 20-hydroxyeicosatetranoic acid (20-HETE). Stimulation by 10(-6) M ANG II was abolished by PLC and protein kinase C (PKC) blockade and was partially suppressed when the rise in cytosolic Ca2+ was prevented. All ANG II effects were abolished by DUP-753 (losartan) but not by PD-123319. Thus less than or equal to 10(-12) MANG II inhibits via 20-HETE, whereas greater than or equal to 5 x 10(-11) M ANG II stimulates via PKC Na+-K+(NH4+)-2C1(-) cotransport; all ANG II effects involve AT(1) receptors and PLC activation.
引用
收藏
页码:C1047 / C1056
页数:10
相关论文
共 30 条
[1]   Na+-K+(NH4+)-2Cl(-) cotransport in medullary thick ascending limb: Control by PKA, PKC, and 20-HETE [J].
Amlal, H ;
Legoff, C ;
Vernimmen, C ;
Paillard, M ;
Bichara, M .
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 1996, 271 (02) :C455-C463
[2]  
AMLAL H, 1994, J BIOL CHEM, V269, P21962
[3]   CL--DEPENDENT NH4+ TRANSPORT MECHANISMS IN MEDULLARY THICK ASCENDING LIMB CELLS [J].
AMLAL, H ;
PAILLARD, M ;
BICHARA, M .
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 1994, 267 (06) :C1607-C1615
[4]   CAMP-DEPENDENT CONTROL OF NA+/H+ ANTIPORT BY AVP, PTH, AND PGE2 IN RAT MEDULLARY THICK ASCENDING LIMB CELLS [J].
BORENSZTEIN, P ;
JUVIN, P ;
VERNIMMEN, C ;
POGGIOLI, J ;
PAILLARD, M ;
BICHARA, M .
AMERICAN JOURNAL OF PHYSIOLOGY, 1993, 264 (02) :F354-F364
[5]   RT-PCR ANALYSIS OF NA+/H+ EXCHANGER MESSENGER-RNAS IN RAT MEDULLARY THICK ASCENDING LIMB [J].
BORENSZTEIN, P ;
FROISSART, M ;
LAGHMANI, K ;
BICHARA, M ;
PAILLARD, M .
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, 1995, 268 (06) :F1224-F1228
[6]   PROXIMAL TUBULAR SECRETION OF ANGIOTENSIN-II IN RATS [J].
BRAAM, B ;
MITCHELL, KD ;
FOX, J ;
NAVAR, LG .
AMERICAN JOURNAL OF PHYSIOLOGY, 1993, 264 (05) :F891-F898
[7]  
DOUGLAS JG, 1994, ANNU REV PHYSIOL, V56, P649
[8]   CYTOCHROME-P-450 ARACHIDONATE METABOLITES AFFECT ION FLUXES IN RABBIT MEDULLARY THICK ASCENDING LIMB [J].
ESCALANTE, B ;
ERLIJ, D ;
FALCK, JR ;
MCGIFF, JC .
AMERICAN JOURNAL OF PHYSIOLOGY, 1994, 266 (06) :C1775-C1782
[9]  
GAMBA G, 1994, J BIOL CHEM, V269, P17713
[10]  
GARVIN J L, 1988, American Journal of Physiology, V255, pF57