Preferential uptake of long chain polyunsaturated fatty acids by isolated human placental membranes

被引:89
作者
Campbell, FM [1 ]
Gordon, MJ [1 ]
DuttaRoy, AK [1 ]
机构
[1] ROWETT RES INST, ABERDEEN AB2 9SB, SCOTLAND
关键词
human placenta; fatty acid uptake; essential fatty acids; plasma membrane fatty acid-binding protein (FABP(pm)); long chain polyunsaturated fatty acids; fetal development;
D O I
10.1007/BF00714336
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Fatty acid uptake by the placenta is thought to be a carrier-mediated process, however the mechanism by which long chain polyunsaturated fatty acids (LCPUFA) are preferentially accumulated from the maternal circulation to the fetal tissues is still unclear. To examine the role of the placenta in this process, binding of four different radiolabelled fatty acids ([C-14]oleate, [C-14]linoleate, [C-14]a-linolenate and [C-14]arachidonate) to human placental membranes was studied. Binding of fatty acid was found to be time- and temperature dependent. At equilibrium, the total binding of oleate was highest (5.1 +/- 0.1 nmoles/mg protein) followed by linoleate (2.8 +/- 0.31 nmoles/mg protein) and arachidonate (2.06 +/- 0.4 nmoles/mg protein) and alpha-linolenate binding was lowest (0.5 + 0.1 nmoles/mg protein). However, oleate had the lowest specific binding (37% of the total binding) whereas arachidonate had the highest specific binding (similar to 86% of the total binding) followed by linoleate and a-linolenate (62%, and 69% of the total binding, respectively). Binding of each [C-14] fatty acid was also assessed in the presence of 20-fold excess of other unlabelled ligands. Binding sites seem to have preference for the binding of [C-14] fatty acids in the following order: arachidonic acid >>> linoleic acid >> a-linolenic acid >>>>> oleic acid, whereas BSP and a-tocopherol did not show any competition with any of the [C-14] fatty acids. These data suggest that the fatty acid binding sites in placental membranes are specific for the fatty acids but that they have heterogeneous affinities. Trans fatty acids (elaidic and linoelaidic acids) also competed very strongly for the [C-14] fatty acid binding. Polyclonal antiserum raised against placental FABP(pm) inhibited binding of these [C-14]fatty acids but with variable degrees of inhibition; EFA/LCPUFA binding was much more than that of oleate. Our data suggest that EFA/LCPUFA bound to albumin are preferentially transported by human placental membranes and that the placental FABP(pm) may be involved in the sequestration of EFA/LCPUFA by the placenta.
引用
收藏
页码:77 / 83
页数:7
相关论文
共 41 条
[1]  
ABUMRAD NA, 1984, J BIOL CHEM, V259, P8945
[2]  
Bereziat G, 1992, ESSENTIAL FATTY ACID, P45
[3]   ALTERATIONS IN THE FATTY-ACID COMPOSITION OF RAT-BRAIN CELLS (NEURONS, ASTROCYTES, AND OLIGODENDROCYTES) AND OF SUBCELLULAR-FRACTIONS (MYELIN AND SYNAPTOSOMES) INDUCED BY A DIET DEVOID OF N-3 FATTY-ACIDS [J].
BOURRE, JM ;
PASCAL, G ;
DURAND, G ;
MASSON, M ;
DUMONT, O ;
PICIOTTI, M .
JOURNAL OF NEUROCHEMISTRY, 1984, 43 (02) :342-348
[4]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[5]   PLASMA-MEMBRANE FATTY-ACID-BINDING PROTEIN (FABP(PM)) OF THE SHEEP PLACENTA [J].
CAMPBELL, FM ;
GORDON, MJ ;
DUTTAROY, AK .
BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM, 1994, 1214 (02) :187-192
[6]   PLASMA-MEMBRANE FATTY-ACID-BINDING PROTEIN IN HUMAN PLACENTA - IDENTIFICATION AND CHARACTERIZATION [J].
CAMPBELL, FM ;
TAFFESSE, S ;
GORDON, MJ ;
DUTTAROY, AK .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1995, 209 (03) :1011-1017
[7]  
CHAMBAZ J, 1985, BIOL NEONATE, V47, P136, DOI 10.1159/000242104
[8]  
CLANDININ MT, 1980, EARLY HUM DEV, V5, P1
[9]  
COLEMAN RA, 1989, SEMIN PERINATOL, V13, P180
[10]  
CRAWFORD MA, 1981, PROG LIPID RES, V20, P30