Genomics-based selection and functional characterization of triterpene glycosyltransferases from the model legume Medicago truncatula

被引：257

作者：

Achnine, L ^{[1
]}

Huhman, DV ^{[1
]}

Farag, MA ^{[1
]}

Sumner, LW ^{[1
]}

Blount, JW ^{[1
]}

Dixon, RA ^{[1
]}

机构：

[1] Samuel Roberts Noble Fdn Inc, Div Plant Biol, Ardmore, OK 73401 USA

来源：

PLANT JOURNAL | 2005年 / 41卷 / 06期

关键词：

triterpene; saponin; model legume; DNA arrays; E. coli expression;

D O I：

10.1111/j.1365-313X.2005.02344.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The biosynthesis of triterpene saponins is poorly characterized in spite of the importance of these glycosylated secondary metabolites for plant defense and animal health. The model legume Medicago truncatula synthesizes more than 30 different saponins based on at least five triterpene aglycones; soyasapogenols B and E, medicagenic acid, hederagenin and bayogenin. We have employed an inducible cell culture system, DNA array-based and in silico transcript profiling, and targeted metabolite profiling, to identify triterpene glycosyltransferases (GTs) from among the more than 300 GTs expressed in M. truncatula. Two uridine diphosphate glucosyltransferases were functionally characterized; UGT73K1 with specificity for hederagenin and soyasapogenols B and E, and UGT71G1 with specificity for medicagenic acid. The latter enzyme also glycosylated certain isoflavones and the flavonol quercetin with higher efficiency than triterpenes; however, integrated transcript and metabolite profiling supported a function for UGT71G1 in terpenoid but not (iso) flavonoid biosynthesis in the elicited cell cultures.

引用

页码：875 / 887

页数：13

共 51 条

[1] Colocalization of L-phenylalanine ammonia-lyase and cinnarnate 4-hydroxylase for metabolic channeling in phenylpropanoid biosynthesis [J].

Achnine, L ;

Blancaflor, EB ;

Rasmussen, S ;

Dixon, RA .

PLANT CELL, 2004, 16 (11) :3098-3109

[2]

ANHALT S, 1992, PLANTA, V187, P83, DOI 10.1007/BF00201627

[3] Extensive reprogramming of primary and secondary metabolism by fungal elicitor or infection in parsley cells [J].

Batz, O ;

Logemann, E ;

Reinold, S ;

Hahlbrock, K .

BIOLOGICAL CHEMISTRY, 1998, 379 (8-9) :1127-1135

[4] Quillaja saponin formulations that stimulate proinflammatory cytokines elicit a potent acquired cell-mediated immunity [J].

Behboudi, S ;

Morein, B ;

Villacres-Eriksson, MC .

SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 1999, 50 (04) :371-377

[5] URIDINEDIPHOSPHATE-GLUCOSE - ISOVITEXIN 7-O-GLUCOSYLTRANSFERASE FROM BARLEY PROTOPLASTS - SUBCELLULAR-LOCALIZATION [J].

BLUME, DE ;

JAWORSKI, JG ;

MCCLURE, JW .

PLANTA, 1979, 146 (02) :199-202

[6]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[7] Estrogen-like activity of ginsenoside Rg1 derived from Panax notoginseng [J].

Chan, RYK ;

Chen, WF ;

Dong, A ;

Guo, D ;

Wong, MS .

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2002, 87 (08) :3691-3695

[8]

CHURCH GH, 1984, P NATL ACAD SCI USA, V81, P65

[9] DOSE RESPONSES FOR COLLETOTRICHUM-LINDEMUTHIANUM ELICITOR-MEDIATED ENZYME-INDUCTION IN FRENCH BEAN CELL-SUSPENSION CULTURES [J].

DIXON, RA ;

DEY, PM ;

MURPHY, DL ;

WHITEHEAD, IM .

PLANTA, 1981, 151 (03) :272-280

[10] Natural products and plant disease resistance [J].

Dixon, RA .

NATURE, 2001, 411 (6839) :843-847

← 1 2 3 4 5 6 →