A recombinant PCR approach requiring only three non-chimeric primers to generate a minigene of interest

被引：3

作者：

Chotani, MA

Chiu, IM

机构：

[1] OHIO STATE UNIV,DEPT INTERNAL MED,DAVIS MED RES CTR,COLUMBUS,OH 43210

[2] OHIO STATE UNIV,PROGRAM MOLEC CELLULAR & DEV BIOL,COLUMBUS,OH 43210

来源：

GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING | 1996年 / 12卷 / 3-4期

关键词：

recombinant PCR; polymerase chain reaction; FGF-I minigene;

D O I：

10.1016/1050-3862(95)00124-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The recombinant PCR allows construction of chimeric molecules. Here we describe this approach utilizing non-chimeric primers. Unlike previous recombinant PCR methods, this approach eliminates the need of multiple sets of primers and multiple rounds of PCR making it an economical and expeditious alternative. We have used this approach to generate an FGF-1 minigene.

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收藏

页码：133 / 135

页数：3

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