Novel human BTB/POZ domain-containing zinc finger protein ZNF295 is directly associated with ZFP161

被引:17
作者
Wang, J [1 ]
Kudoh, J [1 ]
Takayanagi, A [1 ]
Shimizu, N [1 ]
机构
[1] Keio Univ, Dept Mol Biol, Sch Med, Shinjuku Ku, Tokyo 1608582, Japan
基金
日本学术振兴会;
关键词
BTB/POZ; zinc finger; transcription repressor; chromosome; 21q22.3;
D O I
10.1016/j.bbrc.2004.12.048
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Human ZNF295 protein harbors a BTB/POZ domain and multiple kruppel (C2H2) type zinc finger domains, and thus belongs to a family of POK (POZ and kruppel) transcription factor. We have identified many transcript variants generated by the alternative splicing in 5' non-coding exons, an intra-exonic splicing in a coding region, and the use of three polyadenylation signals in the 3' UTR. The intra-exonic splicing removes 603-bp coding sequence, and thus ZNF295 gene produces two protein isoforms: ZNF295L with 1066 amino acid residues and ZNF295S with 865 amino acid residues, containing 9 and 5 zinc finger domains, respectively. ZNF295 is ubiquitously expressed in human fetal and adult tissues. Analysis of transcription activity of ZNNF-295 using various promoter-reporters demonstrated that ZNF295 acts as a transcription repressor, and contains two separate regions for repression activity: the BTB/POZ domain and the central region between BTB/POZ and ZF domains. Both ZNF295L and ZNF295S could interact not only with themselves and each other, but also with another POK protein ZFP161 known to function as a transcription repressor and an activator. We postulated that ZNF295 may be involved in the bi-directional control of gene expression in concert, with ZFP161. (C) 2004 Elsevier Inc. All rights reserved.
引用
收藏
页码:615 / 627
页数:13
相关论文
共 36 条
[1]   Crystal structure of the BTB domain from PLZF [J].
Ahmad, KF ;
Engel, CK ;
Privé, GG .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (21) :12123-12128
[2]   A comprehensive linkage analysis of chromosome 21q22 supports prior evidence for a putative bipolar affective disorder locus [J].
Aita, VM ;
Liu, JJ ;
Knowles, JA ;
Terwilliger, JD ;
Baltazar, R ;
Grunn, A ;
Loth, JE ;
Kanyas, K ;
Lerer, B ;
Endicott, J ;
Wang, ZY ;
Penchaszadeh, G ;
Gilliam, TC ;
Baron, M .
AMERICAN JOURNAL OF HUMAN GENETICS, 1999, 64 (01) :210-217
[3]  
ALBAGLI O, 1995, CELL GROWTH DIFFER, V6, P1193
[4]   BASIC LOCAL ALIGNMENT SEARCH TOOL [J].
ALTSCHUL, SF ;
GISH, W ;
MILLER, W ;
MYERS, EW ;
LIPMAN, DJ .
JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410
[5]   THE POZ DOMAIN - A CONSERVED PROTEIN-PROTEIN INTERACTION MOTIF [J].
BARDWELL, VJ ;
TREISMAN, R .
GENES & DEVELOPMENT, 1994, 8 (14) :1664-1677
[6]   Prediction of complete gene structures in human genomic DNA [J].
Burge, C ;
Karlin, S .
JOURNAL OF MOLECULAR BIOLOGY, 1997, 268 (01) :78-94
[7]   Categorization and characterization of transcript-confirmed constitutively and alternatively spliced introns and exons from human [J].
Clark, F ;
Thanaraj, TA .
HUMAN MOLECULAR GENETICS, 2002, 11 (04) :451-464
[8]   All in the family: the BTB/POZ, KRAB, and SCAN domains [J].
Collins, T ;
Stone, JR ;
Williams, AJ .
MOLECULAR AND CELLULAR BIOLOGY, 2001, 21 (11) :3609-3615
[9]   Novel BTB/POZ domain zinc-finger protein, LRF, is a potential target of the LAZ-3/BCL-6 oncogene [J].
Davies, JM ;
Hawe, N ;
Kabarowski, J ;
Huang, QH ;
Zhu, J ;
Brand, NJ ;
Leprince, D ;
Dhordain, P ;
Cook, M ;
Morriss-Kay, G ;
Zelent, A .
ONCOGENE, 1999, 18 (02) :365-375
[10]   Colocalization and heteromerization between the two human oncogene POZ/zinc finger proteins, LAZ3 (BCL6) and PLZF [J].
Dhordain, P ;
Albagli, O ;
Honore, N ;
Guidez, F ;
Lantoine, D ;
Schmid, M ;
De The, H ;
Zelent, A ;
Koken, MHM .
ONCOGENE, 2000, 19 (54) :6240-6250