Comparative proteomic analysis of human whole saliva

被引:151
作者
Huang, CM [1 ]
机构
[1] Natl Sun Yat Sen Univ, Ctr Neurosci, Kaohsiung 80424, Taiwan
关键词
human saliva; proteomic; two-dimensional gel electrophoresis; mass spectrometry;
D O I
10.1016/j.archoralbio.2004.06.003
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Human saliva performs a wide variety of biological functions that are critical for the maintenance of the oral health. Various functions include Lubrication, buffering, antimicrobial protection, and the maintenance of mucosal. integrity. In addition, whole saliva may be analysed for the diagnosis of human systemic diseases, since it can be readily collected and contains identifiable serum constituents. By using proteomic approach, we have established a reference proteome map of human whole sativa allowing for the resolution of greater than 200 protein spots in a single two-dimensional polyacrylamide get. Fifty-four protein spots, comprised of 26 different proteins, were identifies using N-terminal. sequencing, mass spectrometry, and/or computer matching with protein database. Ten proteins, whose levels were significantly different when bleeding had occurred in the oral cavity, were discussed in this study. These 10 proteins include alpha-1-antrypsin, apolipoprotein A-I, cystatin A, SA, SAIII, and SN, enolase I, hemoglobin beta-chain, thioredoxin peroxiredoxin B, as well as a protactin-inducible protein. The proteomic approach identifies candidates from human whole saliva that may prove to be of diagnostic and therapeutic significance. (C) 2004 Elsevier Ltd. ALL rights reserved.
引用
收藏
页码:951 / 962
页数:12
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