Evidence for copper homeostasis function metallothionein of metallothionein (MT3) in the hyperaccumulator Thlaspi caerulescens

被引：76

作者：

Roosens, NH

Bernard, C

Leplae, R

Verbruggen, N

机构：

[1] Free Univ Brussels, Lab Physiol & Genet Mol Plantes, B-1050 Brussels, Belgium

[2] Free Univ Brussels, Serv Conformat Macromol Biol & Bioinformat, B-1050 Brussels, Belgium

来源：

FEBS LETTERS | 2004年 / 577卷 / 1-2期

关键词：

heavy metal stress; cadmium; metallothionein; metal homeostasis; metal hyperaccumulation;

D O I：

10.1016/j.febslet.2004.08.084

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Metallothioneins chelate metals and consequently may be a control point of metal homeostasis. Homologous to type 3 metallothioneins, TcMT3 cDNA was identified in the Cd/ Zn hyperaccumulator, Thlaspi caerulescens. TcMT3 amino acid sequence showed modifications in the Cys positions when compared with its Arabidopsis ortbologue. A structural model established that the MT3 carboxyterminal domain is similar to the 0 domain of animal metallothioneins and predicts a smaller cavity to chelate metals for A. thaliana than for T. caerulescens. Functional testing in yeast and Northern blot analysis added further evidence for adaptative variations of MT3 for the maintenance of Cu homeostasis in a metal hyperaccumulator. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：9 / 16

页数：8

共 33 条

[1] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].