Oxidative stress associated hepatic and renal toxicity induced by ricin in mice

Ricin a glycoprotein from the Ricinus communis seeds, is known to have diverse toxic effects on cells of different visceral organs. We have studied the hepatotoxicity, nephrotoxicity, and oxidative stress following i.p. administration of ricin (25 mug/kg) in Swiss albino male mice. The results of this study revealed that activities of various enzymes like glutamic pyruvic transaminase (GPT), alkaline phosphatase (ALP), gamma glutamyl transpeptidase (gamma-GT), and lactate dehydrogenase (LDH) were increased in plasma, liver, and kidney tissues indicating damage in liver and kidney. Blood urea level was also increased. However, blood creatinine and bilirubin were not altered. Lipid peroxidation increased to 49 and 25% in hepatic and renal tissue. Total non-protein sulfhydryl content decreased in plasma (12%), hepatic (29%), and renal (16%) tissues. Superoxide dismutase activity decreased significantly in liver (43%) and kidney (37%). The activity of glutatione peroxidase was also decreased. The decrease was more prominent in kidney than liver. A significant increase, 20 to 27% in the activity of catalase was observed in plasma, liver, and kidney. These results indicate that ricin produces hepatoxicity, nephrotoxicity, and oxidative damage at 24 h of post treatment. The hepatotoxicity was more prominent than nephrotoxicity. (C) 2003 Elsevier Science Ltd. All rights reserved.