VEGF protects human cerebral hybrid neurons from in vitro ischemia

被引：34

作者：

Han, MK

Kim, M ^{[1
]}

Bae, SY

Kang, L

Han, SY

Lee, YS

Rha, JH

Kim, SU

Roh, JK

机构：

[1] Seoul Univ, Sch Med, Dept Neurol, Seoul 110744, South Korea

[2] Inha Univ, Sch Med, Dept Neurol, Inchon, South Korea

[3] Ajou Univ, Sch Med, Brain Dis Res Ctr, Suwon 441749, South Korea

[4] Univ British Columbia, Dept Med, Div Neurol, Vancouver, BC V6T 1W5, Canada

来源：

NEUROREPORT | 2004年 / 15卷 / 05期

关键词：

human hybrid neuronal cell line; hypoxia; neuroprotective; vascular endothelial growth factor (VEGF); vascular endothelial growth factor receptor-2 (VEGFR-2);

D O I：

10.1097/00001756-200404090-00022

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Vascular endothelial growth factor (VEGF), the most potent angiogenic peptide, protects the neurons against experimental ischemia. However, its neuroprotective effect on human brain is unknown. The present study attempted to determine whether VEGF can protect human cerebral neurons in vitro. AI human hybrid clonal neurons (human cerebral neuron + neuroblastoma cell) were exposed to hypoxia with glucose deprivation. Pretreatment with VEGF reduced the Al cell death, and VEGFR-2/Flk-I and VEGF increased with a neuroprotective effect. However, the human neuroblastoma or neuroglioma cells failed to show these findings. Our results suggest that VEGF can protect human cerebral neurons from cell death after an ischemic insult in vitro, which is correlated to both increased expression of VEGFR-2/Flk-I and VEGF within the cells.

引用

页码：847 / 850

页数：4

共 17 条

[1] Astrocyte-derived VEGF mediates survival and tube stabilization of hypoxic brain microvascular endothelial cells in vitro [J].