Inhibitor sensitivity of respiratory complex I in human platelets: A possible biomarker of ageing

被引：47

作者：

Pich, MM

Bovina, C

Formiggini, G

Cometti, GG

Ghelli, A

Castelli, GP

Genova, ML

Marchetti, M

Semeraro, S

Lenaz, G

机构：

[1] UNIV BOLOGNA,DEPT BIOCHEM G MORRUZZI,I-40126 BOLOGNA,ITALY

[2] S ORSOLA M MALPIGHI HOSP,GERIATR DEPT 5,BOLOGNA,ITALY

[3] UNIV BOLOGNA,DEPT BIOL,I-40126 BOLOGNA,ITALY

来源：

FEBS LETTERS | 1996年 / 380卷 / 1-2期

关键词：

ageing; platelet mitochondrion; complex I; rotenone sensitivity;

D O I：

10.1016/0014-5793(96)00037-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

NADH-Coenzyme Q reductase was assayed in platelet mitochondrial membranes obtained from 19 pools of two venous blood samples from female young (19-30 years) individuals and 18 pools from aged ones (66-107 years). The enzyme activities were not significantly changed in the two groups, but a decrease of sensitivity to the specific inhibitor, rotenone, occurred in a substantial number of aged individuals, The results are in agreement with the predictions of the mitochondrial theory of ageing and may be used to develop a sensitive biomarker of the ageing process.

引用

收藏

页码：176 / 178

页数：3

相关论文

共 22 条

[1] AGE-RELATED-CHANGES IN THE HEMOSTATIC SYSTEM [J].

ABBATE, R ;

PRISCO, D ;

ROSTAGNO, C ;

BODDI, M ;

GENSINI, GF .

INTERNATIONAL JOURNAL OF CLINICAL & LABORATORY RESEARCH, 1993, 23 (01) :1-3

[2]

BLASS JP, 1977, CLIN CHIM ACTA, V74, P21

[3] MITOCHONDRIAL ACTIVITIES OF RAT-HEART DURING AGING [J].

CASTELLUCCIO, C ;

BARACCA, A ;

FATO, R ;

PALLOTTI, F ;

MARANESI, M ;

BARZANTI, V ;

GORINI, A ;

VILLA, RF ;

CASTELLI, GP ;

MARCHETTI, M ;

LENAZ, G .

MECHANISMS OF AGEING AND DEVELOPMENT, 1994, 76 (2-3) :73-88

[4] ANALYSES OF MITOCHONDRIAL RESPIRATORY-CHAIN FUNCTION AND MITOCHONDRIAL-DNA DELETION IN HUMAN SKELETAL-MUSCLE - EFFECT OF AGING [J].

COOPER, JM ;

MANN, VM ;

SCHAPIRA, AHV .

JOURNAL OF THE NEUROLOGICAL SCIENCES, 1992, 113 (01) :91-98

[5] ASSOCIATION OF MITOCHONDRIAL-DNA DAMAGE WITH AGING AND CORONARY ATHEROSCLEROTIC HEART-DISEASE [J].

CORRALDEBRINSKI, M ;

SHOFFNER, JM ;

LOTT, MT ;

WALLACE, DC .

MUTATION RESEARCH, 1992, 275 (3-6) :169-180

[6]

DIMAURO S, CT ARCH NEUROL, V50, P1197

[7] NATURAL SUBSTANCES (ACETOGENINS) FROM THE FAMILY ANNONACEAE ARE POWERFUL INHIBITORS OF MITOCHONDRIAL NADH DEHYDROGENASE (COMPLEX-I) [J].

ESPOSTI, MD ;

GHELLI, A ;

RATTA, M ;

CORTES, D ;

ESTORNELL, E .

BIOCHEMICAL JOURNAL, 1994, 301 :161-167

[8] THE MECHANISM OF PROTON AND ELECTRON-TRANSPORT IN MITOCHONDRIAL COMPLEX-I [J].

ESPOSTI, MD ;

GHELLI, A .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1994, 1187 (02) :116-120

[9] FUNCTIONAL ALTERATIONS OF THE MITOCHONDRIALLY ENCODED ND4 SUBUNIT ASSOCIATED WITH LEBERS HEREDITARY OPTIC NEUROPATHY [J].

ESPOSTI, MD ;

CARELLI, V ;

GHELLI, A ;

RATTA, M ;

CRIMI, M ;

SANGIORGI, S ;

MONTAGNA, P ;

LENAZ, G ;

LUGARESI, E ;

CORTELLI, P .

FEBS LETTERS, 1994, 352 (03) :375-379

[10] COMPLEX-I AND COMPLEX-III OF MITOCHONDRIA HAVE COMMON INHIBITORS ACTING AS UBIQUINONE ANTAGONISTS [J].

ESPOSTI, MD ;

GHELLI, A ;

CRIMI, M ;

ESTORNELL, E ;

FATO, R ;

LENAZ, G .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1993, 190 (03) :1090-1096