Total and light fraction organic C in a thin Black Chernozemic grassland soil as affected by 27 annual applications of six rates of fertilizer N

Maintenance and sequestration of C is important to sustain and improve the quality and productivity of soils. The objective of this study was to determine the effects of 27 annual applications of six N rates ( 0, 56, 112, 168, 224 and 336 kg N ha(-1) yr(-1)) on total organic C (TOC) and light fraction organic C (LFOC) in a thin Black Chernozemic loam soil. Nitrogen (ammonium nitrate) was surface-applied to bromegrass (Bromus inermis Leyss) managed as hay near Crossfield, Alberta, Canada. The concentration and mass of TOC and LFOC in the 0-5, 5-10, 10-15 and 15-30 cm soil layers increased with N rate and showed a quadratic response to N rate with significant R-2 values, with their maximum values at 336 kg N ha(-1) in the 0-5 cm layer and at 224 kg N ha(-1) in other layers. But the increase in TOC and LFOC per kg of N addition was maximized at 56 kg N ha(-1) and declined with further increase in N rate. These trends indicated that higher N rates would cause a faster build up of soil C, whereas lower N rates would achieve a greater increase in soil C per unit of N addition. Response of C mass to N application was much greater for LFOC (range of 697 to 156% increase) than for TOC (range of 67 to 17% increase). Percentage of LFOC in TOC mass increased with N rate. At the 168 to 336 kg N ha(-1) rates, almost all of the increase in TOC in the surface 10 cm soil occurred as LFOC. Thus, LFOC was more responsive to N application and was a good indicator of N effect on soil C. The trend of change in soil TOC and LFOC was similar to hay yield and C removal in hay, which suggests that increasing hay yield with N application concurrently also increases soil organic C. In conclusion, long-term annual applications of N fertilizer to bromegrass resulted in a substantial increase in TOC and LFOC in the soil, thereby indicating that N fertilization can be used to sequester more atmospheric C in prairie grassland soils.