Suppression of microRNA-silencing pathway by HIV-1 during virus replication

被引：502

作者：

Triboulet, Robinson

Mari, Bernard

Lin, Yea-Lih

Chable-Bessia, Christine

Bennasser, Yamina

Lebrigand, Kevin

Cardinaud, Bruno

Maurin, Thomas

Barbry, Pascal

Baillat, Vincent

Reynes, Jacques

Corbeau, Pierre

Jeang, Kuan-Teh

Benkirane, Monsef ^{[1
]}

机构：

[1] Inst Genet Humaine, Mol Virol Lab, Montpellier, France

[2] Inst Genet Humaine, Lab Lentivirus & Transfert Genes, Montpellier, France

[3] UNSA, CNRS, UMR 6097, Inst Pharmacol Mol & Cellulaire, Sophia Antipolis, France

[4] Hop Gui de Chauliac, Serv Malad Infect & Trop, Montpellier, France

[5] NIAID, Mol Microbiol Lab, NIH, Bethesda, MD 20892 USA

来源：

SCIENCE | 2007年 / 315卷 / 5818期

关键词：

D O I：

10.1126/science.1136319

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

MicroRNAs ( miRNAs) are single-stranded noncoding RNAs of 19 to 25 nucleotides that function as gene regulators and as a host cell defense against both RNA and DNA viruses. We provide evidence for a physiological role of the miRNA-silencing machinery in controlling HIV-1 replication. Type III RNAses Dicer and Drosha, responsible for miRNA processing, inhibited virus replication both in peripheral blood mononuclear cells from HIV-1-infected donors and in latently infected cells. In turn, HIV-1 actively suppressed the expression of the polycistronic miRNA cluster miR-17/92. This suppression was found to be required for efficient viral replication and was dependent on the histone acetyltransferase Tat cofactor PCAF. Our results highlight the involvement of the miRNA-silencing pathway in HIV-1 replication and latency.

引用

页码：1579 / 1582

页数：4

共 22 条

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