Cell orientation determines the alignment of cell-produced collagenous matrix

被引:215
作者
Wang, JHC [1 ]
Jia, FY [1 ]
Gilbert, TW [1 ]
Woo, SLY [1 ]
机构
[1] Univ Pittsburgh, Med Ctr, Musculoskeletal Res Ctr, Dept Orthopaed Surg, Pittsburgh, PA 15213 USA
关键词
MC3T3-E1; cells; microgrooves; orientation; collagen matrix; alignment;
D O I
10.1016/S0021-9290(02)00233-6
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
In healing ligaments and tendons, the cells are not aligned and collagen matrix is not organized as in normal tissues. In addition, the mechanical properties of the tissues are abnormal. We hypothesized that the lack of alignment of the collagen matrix results from random orientation of the cells seen in the healing area. To test this hypothesis, a novel in vitro model was used in which the orientation of cells could be controlled via microgrooves, and alignment of the collagen matrix formed by these cells could be easily observed. It is known that cells align uniformly along the direction of microgrooves; therefore MC3T3-E1 cells, which produce large amounts of collagen, were grown on silicone membranes with parallel microgrooves (10 mum wide x 3 mum deep) in the surface. As a control, the same cells were also grown on smooth silicone membranes. Cells on both the microgrooved and smooth silicone surfaces produced a layer of readily visible collagen matrix. Immunohistochemical staining showed that the matrix consisted of abundant type I collagen. Polarized light microscopy of the collagen matrix revealed the collagen fibers to be parallel to the direction of the microgrooves, whereas the collagen matrix produced by the randomly oriented cells on the smooth membranes was disorganized. Thus, the results of this study suggest that the orientation of cells affects the organization of the collagenous matrix produced by the cells. The results also suggest that orienting cells along the longitudinal direction of healing ligaments and tendons may lead to the production of aligned collagenous matrix that more closely represents the uninjured state. This may enhance the mechanical properties of healing ligaments and tendons. (C) 2002 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:97 / 102
页数:6
相关论文
共 38 条
[1]   PRODUCTION OF A TISSUE-LIKE STRUCTURE BY CONTRACTION OF COLLAGEN LATTICES BY HUMAN-FIBROBLASTS OF DIFFERENT PROLIFERATIVE POTENTIAL INVITRO [J].
BELL, E ;
IVARSSON, B ;
MERRILL, C .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (03) :1274-1278
[2]   Production of ordered collagen matrices for three-dimensional cell culture [J].
Besseau, L ;
Coulomb, B ;
Lebreton-Decoster, C ;
Giraud-Guille, MM .
BIOMATERIALS, 2002, 23 (01) :27-36
[3]   EXTRACELLULAR COMPARTMENTS IN TENDON MORPHOGENESIS - COLLAGEN FIBRIL, BUNDLE, AND MACROAGGREGATE FORMATION [J].
BIRK, DE ;
TRELSTAD, RL .
JOURNAL OF CELL BIOLOGY, 1986, 103 (01) :231-240
[4]  
Brown RA, 1998, J CELL PHYSIOL, V175, P323, DOI 10.1002/(SICI)1097-4652(199806)175:3<323::AID-JCP10>3.0.CO
[5]  
2-6
[6]   The effects of the surface topography of micromachined titanium substrata on cell behavior in vitro and in vivo [J].
Brunette, DM ;
Chehroudi, B .
JOURNAL OF BIOMECHANICAL ENGINEERING-TRANSACTIONS OF THE ASME, 1999, 121 (01) :49-57
[7]   Topographical control of cells [J].
Curtis, A ;
Wilkinson, C .
BIOMATERIALS, 1997, 18 (24) :1573-1583
[8]  
Eastwood M, 1998, CELL MOTIL CYTOSKEL, V40, P13, DOI 10.1002/(SICI)1097-0169(1998)40:1<13::AID-CM2>3.0.CO
[9]  
2-G
[10]  
FRANK GJ, 1983, J INT MED RES, V11, P6