Characterisation of fluorescent Schiff bases formed during oxidation of pig myofibrils

被引:80
作者
Chelh, Ilham [1 ]
Gatellier, Philippe [1 ]
Sante-Lhoutellier, Veronique [1 ]
机构
[1] INRA, Ctr Theix, Qualite Produits Animaux, F-63122 St Genes Champanelle, France
关键词
myofibrils; protein oxidation; carbonyls; lipid oxidation; TBARS; front-face fluorescence; fluorescent pigments;
D O I
10.1016/j.meatsci.2006.10.028
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
The aim of this study was to investigate the formation of fluorescent Schiff bases between proteins and lipid oxidation products in myofibrils. Myofibrils were prepared from pig M. longissimus dorsi and oxidized by hydroxyl (OH center dot) and superoxide (O-2(center dot-)) radical generating systems. Protein oxidation was measured by the carbonyl content and lipid oxidation was estimated by measurement of thiobarbituric acid reactive substances (TBARS). To avoid any bias due to their low solubility, fluorescent pigments were estimated directly in the solid state by a front-face fluorescence technique. Hydroxyl radicals generated high levels of lipid and protein oxidation as well as fluorescent pigments, whereas only fluorescence was affected by superoxide radicals. The formation of fluorescent pigments was linked not only to aldehyde production, but also to the availability of the amino groups of the myofibrillar protein side chains. Schiff bases could be implicated in protein aggregation with deleterious effect on meat quality. (c) 2006 Elsevier Ltd. All rights reserved.
引用
收藏
页码:210 / 215
页数:6
相关论文
共 37 条
[1]  
ASGHAR A, 1988, FOOD TECHNOL-CHICAGO, V42, P102
[2]   A COMPARATIVE-STUDY ON THE EFFECTS OF AGING AND TRAINING ON THE LEVELS OF LIPOFUSCIN IN VARIOUS TISSUES OF THE RAT [J].
BASSON, ABK ;
TERBLANCHE, SE ;
OELOFSEN, W .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 1982, 71 (03) :369-374
[3]   THE PATHOLOGY OF THE SKELETAL AND CARDIAC MUSCLES OF CATTLE WITH XANTHOSIS [J].
BRADLEY, R ;
DUFFELL, SJ .
JOURNAL OF COMPARATIVE PATHOLOGY, 1982, 92 (01) :85-97
[4]  
DAVIES KJA, 1987, J BIOL CHEM, V262, P9895
[5]  
DAVIES KJA, 1987, J BIOL CHEM, V262, P9902
[6]  
FRIDOVICH I, 1970, J BIOL CHEM, V245, P4053
[7]  
FRIGUET B, 1994, J BIOL CHEM, V269, P21639
[8]   Effect of a dietary vitamin E supplementation on colour stability and lipid oxidation of air- and modified atmosphere-packaged beef [J].
Gatellier, P ;
Hamelin, C ;
Durand, Y ;
Renerre, M .
MEAT SCIENCE, 2001, 59 (02) :133-140
[9]  
GORNALL AG, 1949, J BIOL CHEM, V177, P751
[10]   Decreased proteolysis caused by protein aggregates, inclusion bodies, plaques, lipofuscin, ceroid, and 'aggresomes' during oxidative stress, aging, and disease [J].
Grune, T ;
Jung, T ;
Merker, K ;
Davies, KJA .
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2004, 36 (12) :2519-2530