Proliferative heterogeneity in the human prostate: Evidence for epithelial stem cells

被引:121
作者
Hudson, DL
O'Hare, M
Watt, FM
Masters, JRW
机构
[1] Univ London, Univ Coll London, Sch Med, Inst Urol & Nephrol,Res Labs, London W1P 7PN, England
[2] Univ London, Univ Coll London, Sch Med, Dept Surg,LICR UCL Breast Canc Lab, London W1P 7PN, England
[3] Imperial Canc Res Fund, Keratinocyte Lab, London WC2A 3PX, England
关键词
D O I
10.1038/labinvest.3780132
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Clonal analysis of human prostate epithelial cells was undertaken in order to identify stem cells. Two types of colony were distinguished, termed type I and type II. Type I colonies were relatively small and irregular and contained a loose mixture of differentiated and undifferentiated cells. In contrast, type II colonies were large, round, and homogeneous, consisting almost exclusively of small undifferentiated and dividing cells. The colony-forming efficiency was 5.8% +/- 1.8 for freshly isolated epithelial cells. There were approximately 10 times as many type I as type II colonies and about 1 in 200 of the plated cells was capable of forming a type II colony. In three-dimensional culture on Matrigel, the type II colonies produced structures reminiscent of prostate epithelium, with luminal cells expressing markers of prostate epithelial differentiation, including the androgen receptor. On the basis of their proliferative characteristics and pluripotency, the type II colonies may be the progeny of stem cells and the type I colonies of a more differentiated transit-amplifying population.
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页码:1243 / 1250
页数:8
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