A pumpkin 72-kDa membrane protein of precursor-accumulating vesicles has characteristics of a vacuolar sorting receptor

被引：100

作者：

Shimada, T

Kuroyanagi, M

Nishimura, M

Hara-Nishimura, I ^{[1
]}

机构：

[1] Natl Inst Basic Biol, Dept Cell Biol, Okazaki, Aichi 444, Japan

[2] Grad Univ Adv Studies, Sch Life Sci, Dept Mol Biomech, Okazaki, Aichi 444, Japan

来源：

PLANT AND CELL PHYSIOLOGY | 1997年 / 38卷 / 12期

关键词：

EGF-like motif; precursor-accumulating vesicle (PAC vesicle); pumpkin (Cucurbita sp.); sorting receptor; targeting signal; vacuolar protein;

D O I：

10.1093/oxfordjournals.pcp.a029138

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Precursor-accumulating (PAC) vesicles were previously shown to mediate the transport of the precursor of a major storage protein (pro2S albumin) to protein-storage vacuoles in developing pumpkin cotyledons. In this study, we characterized two homologous proteins from PAC vesicles, a 72 kDa protein (PV72) and an 82 kDa protein (PV82). PV72 and PV82 shouted an ability to bind to peptides derived from both an internal propeptide and a C-terminal peptide of pro2S albumin. PV72 was predicted to be a type I integral membrane protein with epidermal growth factor (EGF)-like motifs. These results suggest that PV72 and PV82 are potential sorting receptors fur 2S albumin to protein-storage vacuoles.

引用

页码：1414 / 1420

页数：7

共 23 条

[1] Cloning and subcellular location of an Arabidopsis receptor-like protein that shares common features with protein-sorting receptors of eukaryotic cells [J].