Development and application of Multiple-Locus Variable Number of tandem repeat Analysis (MLVA) to subtype a collection of Listeria monocytogenes

被引：54

作者：

Murphy, Mary

Corcoran, Deborah

Buckley, James F.

O'Mahony, Micheal

Whyte, Paul

Fanning, Seamus ^{[1
]}

机构：

[1] Univ Coll Dublin, Ctr Food Safety, Sch Agr, Dublin 4, Ireland

[2] Cork Cty Council, Vet Food Safety Lab, Cork, Ireland

[3] Cork Inst Technol, Mol Diagnost Unit, Bishopstown, Ireland

[4] Cork & Food Safety Author Ireland, Dublin, Ireland

来源：

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY | 2007年 / 115卷 / 02期

关键词：

Listeria monocytogenes; food-borne pathogen; MLVA; subtyping Listeria monocytogenes;

D O I：

10.1016/j.ijfoodmicro.2006.10.022

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

In this study we report the development and application of a Multiple-Locus Variable number of tandem repeat Analysis (MLVA) strategy for subtyping Listeria monocytogenes. Genome profiles of a collection of forty-five food-borne L. monocytogenes isolates were compared using MLVA. These isolates were obtained as part of an active surveillance programme of foods in the south-east region of Ireland. MLVA successfully discriminated amongst the isolates. The method was easy to perform, relatively fast and could be deployed in any molecular laboratory with basic laboratory equipment. This approach is a valuable tool, which has the capability to provide comparable results when compared with other more established typing methods, including pulsed-field gel electrophoresis (PFGE). (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：187 / 194

页数：8

共 28 条

[1] Typing of Listeria monocytogenes isolates originating from the food processing industry with automated ribotyping and pulsed-field gel electrophoresis [J].