Simultaneous changes in the mechanical properties, quantitative collagen organization, and proteoglycan concentration of articular cartilage following canine meniscectomy

被引:108
作者
LeRoux, MA
Arokoski, J
Vail, TP
Guilak, F
Hyttinen, MM
Kiviranta, I
Setton, LA
机构
[1] Duke Univ, Med Ctr, Dept Biomed Engn, Durham, NC 27708 USA
[2] Duke Univ, Med Ctr, Dept Surg, Div Orthopaed Surg, Durham, NC 27708 USA
[3] Kuopio Univ Hosp, Dept Phys Med & Rehabil, SF-70210 Kuopio, Finland
[4] Univ Kuopio, Dept Anat, FIN-70211 Kuopio, Finland
[5] Jyvaskyla Cent Hosp, Dept Surg, Div Orthopaed Surg, Jyvaskyla, Finland
关键词
D O I
10.1002/jor.1100180309
中图分类号
R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学(修复外科学)];
学科分类号
摘要
The mechanical properties and microstructure of articular cartilage from the canine tibial plateau were studied 12 weeks after total medial meniscectomy. The organization of the birefringent collagen network was measured with quantitative polarized light microscopy to determine the thickness and the degree of organization of the superficial and deep zones. The zonal concentration of sulfated glycosaminoglycan was quantified with digital densitometry of safranin-O staining. Equilibrium compressive and shear properties, as well as dynamic shear properties, were measured at sites adjacent to those of microstructural analysis. The results evinced significant loss of cartilage function following meniscectomy, with decreases of 20-50% in the compressive and shear moduli. There was no evidence of alterations in the degree of collagen fibrillar organization, although a complete loss of the surface zone was seen in 60% of the samples that underwent meniscectomy. Meniscectomy resulted in a decreased concentration of sulfated glycosaminoglycan, and significant positive correlations were found between the equilibrium compressive modulus and the glycosaminoglycan content. Furthermore, the shear properties of cartilage correlated directly with collagen fibrillar organization measured at the superficial zone of corresponding sites. These findings demonstrate that meniscectomy leads to impaired mechanical function of articular cartilage, with significant evidence of quantitative correlations between cartilage microstructure and mechanics.
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页码:383 / 392
页数:10
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