Isoelectric properties of the soluble matrices in relation to the chemical composition of some Scleractinian skeletons

被引：33

作者：

Dauphin, Y

Cuif, JP

机构：

来源：

ELECTROPHORESIS | 1997年 / 18卷 / 07期

关键词：

biomineralization; mineralizing matrices; Scleractinian corals;

D O I：

10.1002/elps.1150180726

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Soluble matrices of four Scleractinian skeletons (Madrepora, Favia, Leptastrea and Fungia), were extracted and studied by isoelectric focusing (IEF). The main part of the extracted matrices is acidic. IEF gels are poorly stained or unstained by Coomassie Blue and silver. The positive Alcian Blue staining is indicative of a sulfated and acidic nature of the glycoproteins. Chemical analysis shows high S contents in Scleractinian skeletons. These results are supported by the comparison with IEF and chemical contents of Molluscan shells.

引用

页码：1180 / 1183

页数：4

共 17 条

[1] [Anonymous], PHYSL MOLLUSCA
[2] ACIDIC MACROMOLECULES ASSOCIATED WITH THE MINERAL PHASE OF SCLERACTINIAN CORAL SKELETONS
CONSTANTZ, B
WEINER, S
[J]. JOURNAL OF EXPERIMENTAL ZOOLOGY, 1988, 248 (03): : 253 - 258
[3] CUIF JP, 1997, P 7 INT S FOSS CNID, V92, P129
[4] Dauphin Y, 1996, MAR BIOL, V125, P525
[5] THE COMPOSITIONAL ANALYSIS OF RECENT CEPHALOPOD SHELL CARBOHYDRATES BY FOURIER-TRANSFORM INFRARED SPECTROMETRY AND HIGH-PERFORMANCE ANION-EXCHANGE-PULSED AMPEROMETRIC DETECTION
DAUPHIN, Y
MARIN, F
[J]. EXPERIENTIA, 1995, 51 (03): : 278 - 283
[6] GREGOIRE C, 1972, CHEM ZOOL, V7, P45
[7] GRILLO MC, 1990, RAPP COMM INT MER ME, V32, P21
[8] JANSON JC, 1989, PROTEIN PURIFICATION
[9] Krampitz G., 1976, MECHANISMS MINERALIZ, P155
[10] KRAMPITZ G, 1983, BIOMINERALIZATION BI, P231, DOI DOI 10.1007/978-94-009-7944-4_20

← 1 2 →