A new method to determine DNA sugar conformation from the joint use of 2D and 3D NMR data

被引：3

作者：

Chaoui, M ^{[1
]}

Mauffret, O ^{[1
]}

Lefebvre, A ^{[1
]}

Lescot, E ^{[1
]}

Tevanian, G ^{[1
]}

Fermandjian, S ^{[1
]}

机构：

[1] INST GUSTAVE ROUSSY,URA 147 CNRS,DEPT BIOL & PHARMACOL STRUCT,PR 2,F-94805 VILLEJUIF,FRANCE

来源：

JOURNAL OF BIOMOLECULAR NMR | 1997年 / 10卷 / 02期

关键词：

B-DNA; 3D NOE-NOE; sugar conformation;

D O I：

10.1023/A:1018306210123

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The use of sugar restraints has been proven essential for assessing DNA structures through molecular modeling studies. We present a new method combining 2D (COSY and NOESY) and 3D (NOESY-NOESY) experiments, where constraints on either the phase angles or the difference between phase angles of two residues are obtained from comparison of 2D NOE H1'-H4' intensities and 3D NOE intensities containing the H1'-H4' transfer. All experiments lead to restraints that match, proving the validity of the method.

引用

页码：187 / 192

页数：6

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